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黄金葡萄球菌通过PerR感知人类中性粒细胞,以协调毒素LukAB的表达

Avital Savin^1,2, Exene E Anderson¹, Sophie Dyzenhaus¹

¹Department of Microbiology, New York University Grossman School of Medicine, New York, New York, USA.

Infection and immunity

|January 18, 2024

概括

黄金葡萄球菌使用过氧化物传感器PerR来检测来自人类中性粒细胞的过氧化. 这种感知可以提高LukAB毒性因子的调节,从而增强细菌对免疫细胞的生存能力.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

黄金葡萄球菌是一种重要的病原体,具有众多的毒性因子.
利可西丁LukAB是一种关键的毒性因子,有助于S. aureus感染.
调节lukAB表达,特别是在宿主免疫细胞相互作用期间,仍然不清楚.

研究的目的:

在S. aureus感染人类多态核中性粒细胞 (hPMNs) 期间识别lukAB的转录调节剂.
阐明PerR在lukAB调节中的作用和hPMNs存在时的细菌细胞毒性.

主要方法:

在hPMN感染期间对lukAB的转录调节者的查.
评估PerR对lukAB促进体活性和细菌细胞毒性的影响.
以PerR-依赖的方式评估对过氧化 (H2O2) 暴露的反应.

主要成果:

通过hPMNs诱导lukAB表达,PerR是必不可少的.
在hPMN感染期间,PerR显著上调细菌细胞毒性.
在对H2O2.2的反应中,PerR调解了增加的lukAB促进体活性.

结论:

黄金菌使用PerR来感知hPMNs产生的H2O2.
这种感知机制刺激了lukAB的表达,帮助细菌对抗先天免疫细胞的生存.
PerR将氧化应激感应与致病因子生产联系起来,以确保病原体的生存.

关键词:

卢卡布卢卡布卢卡布在MRSA中,MRSA可能是MRSA.每一个RRR.黄金葡萄球菌黄金葡萄球菌细胞毒素 (cytotoxins) 是一种毒素.中性粒细胞中性粒细胞.形成毛孔的毒素.

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