含有159的卷曲卷曲域对于小鼠†的精子体头和尾部组合是必需的
Tingting Ge1, Lu Yuan1, Linwei Xu1
1Department of Histology and Embryology, School of Medicine, Yangzhou University, Yangzhou, Jiangsu, China.
Biology of reproduction
|January 18, 2024
概括
卷曲卷曲域含蛋白159 (CCDC159) 对于精子发育至关重要,它将精子的头部与尾部固定在一起. 由于精子结构有缺陷,其缺乏会导致男性不孕不育.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 精子生成研究 精子生成研究
背景情况:
- 中心体在精子结构和功能中起着至关重要的作用,特别是在将头部与尾部连接并形成鞭状微管中.
- 头尾合装置 (HTCA) 对于确保精子头尾之间保持稳定的连接至关重要.
研究的目的:
- 研究CCDC159在精子生成中的功能及其在精子结构中的作用.
- 确定CCDC159调节精子发育的分子机制.
主要方法:
- 在小鼠丸中对CCDC159的局部化研究.
- 在小鼠中对Ccdc159的基因淘汰 (KO).
- 酵母二杂交查,以确定CCD159结合蛋白.
- 对Ccdc159 KO试验物的RNA测序分析.
主要成果:
- CCDC159特异地定位在精子体的HTCA上,并包含一个中心体定位信号.
- Ccdc159 KO小鼠表现出接受性精子,异常的鞭毛和男性不孕.
- CCDC159可能通过调节蛋白酸酶PP1活性来调节HTCA组合.
- 在Ccdc159 KO丸中,许多参与男性性质组生成的基因被下调.
结论:
- CCDC159是一种新型的中心体蛋白质,在精子生成过程中对于将精子的头部与尾部固定至关重要.
- CCDC159的功能对于保持精子结构完整性和男性生育能力至关重要.
- 计划进行进一步的基因救援实验,以充分阐明CCDC159的生物功能.
相关概念视频
02:26Conservation of Protein Domains Over Different Proteins
10.9K
Protein domains are small structurally independent units that are part of a single amino acid chain. Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
10.9K
02:50Spindle Assembly
3.7K
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
3.7K
02:02Meiosis II
45.7K
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
45.7K
01:41Spermatogenesis
102.5K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.5K
02:15Condensins
3.5K
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
3.5K
03:09Meiosis I
40.7K
Meiosis is the division of a diploid cell into haploid cells forming sperm and eggs in animals through differentiation. Meiosis I is the first stage of meiosis, where the genetic recombination of homologous chromosomes and the reduction of the ploidy level by half occurs.
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
40.7K