COOLAIR和PRC2在本地化过程中与沉默平行运行FLC
Mathias Nielsen1, Govind Menon2, Yusheng Zhao1
1Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom.
概括
反意义转录 (COOLAIR) 和多抑制复合体2 (PRC2) 协同工作,使阿拉比多普西斯的FLOWERING LOCUS C (FLC) 基因沉默,对寒冷的温度做出反应,以进行强大的表观遗传调节.
科学领域:
- 植物分子生物学 植物分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因调节 基因调节
背景情况:
- 非编码转录会影响染色体结构和环境反应.
- 连接反意义转录,多抑制复合体2 (PRC2) 和表观遗传沉默的精确机制仍然不完全理解.
研究的目的:
- 调查反感应转录 (COOLAIR) 和PRC2在冬季诱导的阿拉比多花C (FLC) 的表观遗传沉默中的相互作用.
- 通过定义这些途径的动态和调节来阐明FLC抑制的基础分子机制.
主要方法:
- 对具有改变反感路径活性的阿拉比多普西斯系的遗传分析.
- 染色体分析以评估表观遗传状态.
- 计算建模来定义监管机制.
主要成果:
- 通过两个并行的途径来抑制FLC:快速的COOLAIR转录介导反应和较慢的PRC2-依赖的表观遗传维护机制.
- COOLAIR和PRC2通路都由NTL8共同调节,NTL8是一种转录调节器,其积累与在低温下缓慢生长期间减少稀释有关.
结论:
- 快速起作用的COOLAIR和缓慢切换的PRC2的联合作用,由温度依赖的生长动态调节,提供了一种灵活但强大的系统,用于针对波动的环境温度对FLC的表观静音.
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