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Updated: Jun 24, 2026

Real-time Live Imaging of T-cell Signaling Complex Formation
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单分子光成像显示CTPS和P5CS的联合组装

Jian Chang1, Weijie Yuan1, Chendi Gao2

  • 1State Key Laboratory of Surface Physics, Shanghai Key Laboratory of Metasurfaces for Light Manipulation, Department of Physics, Fudan University, Shanghai 200433, China.

The journal of physical chemistry. B
|January 18, 2024
PubMed
概括

细胞质蛋白CTP合成酶 (CTPS) 自组装成称为cytoophidia的纤维. 这项研究揭示了CTPS四基体形成这些结构和与P5CS核聚合,为蛋白质动力学提供了洞察力.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...