识别调节表达的MYC内2区域
Van S Tompkins1, Zheng Xue1, Jake M Peterson1
1Roy J. Carver Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa, United States of America.
PloS one
|January 18, 2024
概括
异质核核核糖核蛋白C (HNRNPC) 结合MYC内核2的多U区域,确保精确的拼接和翻译. 破坏这些部位会损害MYC的产生,突出显示HNRNPC在基因表达中的关键作用.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 在RNA分离过程中.
背景情况:
- MYC转录因子对于分化和增殖等细胞过程至关重要.
- 控制MYC前mRNA拼接的精确机制尚不清楚.
- 异质核核核糖核蛋白C (HNRNPC) 是一种关键的RNA结合蛋白.
研究的目的:
- 调查HNRNPC和MYC内部2之间的相互作用.
- 阐明HNRNPC在MYC前mRNA拼接和翻译中的作用.
- 为了确定MYC内部的监管元素2.
主要方法:
- 利用已公布的eCLIP数据来确认HNRNPC的结合点.
- 在MYC内2中对多U区域进行突变分析.
- 分析了对HNRNPC结合,拼接保真性和翻译效率的影响.
- 检查了跨物种识别的结合区域的序列保护.
主要成果:
- 已确认的HNRNPC与MYC内部2的特定多 (U) 区域结合.
- 证明,破坏所有三个多U) 位点显著降低了HNRNPC的结合,并损害了拼接和翻译的准确性.
- 发现这些poly(U) 序列在多个物种中都保持着.
- 确定了一个HNRNPC绑定站点上游的新型监管元素,在删除时可以增强MYC翻译.
结论:
- 在保持MYC前mRNA拼接和翻译忠实性方面,HNRNPC通过其与内基多元U) 区域的相互作用,发挥了关键的补偿作用.
- 已识别的保存序列和调控元素为理解和潜在地操纵MYC基因表达提供了新的目标.
相关概念视频
01:23Regulation of Expression at Multiple Steps
913
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
913
02:24Regulation of Expression Occurs at Multiple Steps
3.0K
3.0K
02:41Chromatin Structure Regulates pre-mRNA Processing
7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
7.0K
02:02Cis-regulatory Sequences
3.0K
3.0K
02:23Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
01:58Cell Specific Gene Expression
4.6K
4.6K