针对新出现的猪病原体Mycoplasma hyosynoviae的多部位序列类型化方案

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Moritz Bünger¹, Michael Blümlinger¹, Igor Loncaric²

¹University of Veterinary Medicine Vienna, University Clinic for Swine, Veterinärplatz 1, 1210 Vienna, Austria.

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|January 18, 2024

概括

猪病原体Mycoplasma hyosynoviae的新类型化方法揭示了高菌株多样性. 核心基因组 (cg) MLST和常规MLST都对流行病学研究有效,cgMLST为密切相关的分离物提供更高分辨率.

科学领域:

兽医微生物学兽医微生物学
基因组学就是基因组学.
流行病学流行病学

背景情况:

菌菌 (M. hyosynoviae) 是一种猪病原体,引起关节炎.
对M.hyosynoviae的流行病学研究受到缺乏有效的类型化方法的限制.

研究的目的:

开发和比较传统的多部位序列类型 (MLST) 和核心基因组 (cg) MLST方案,用于M.hyosynoviae.
评估M. hyosynoviae菌株的遗传多样性和流行病学相关性.

主要方法:

开发使用全基因组序列的390个点基因cgMLST方案.
为传统的MLST选择七个家庭基因.
使用cgMLST和常规MLST,对73种M. hyosynoviae菌株进行类型化.

主要成果:

cgMLST在M.hyosynoviae菌株中发现了高度的遗传多样性.
确定了7个克隆群,包括20个单独物种.
这两种方法都产生了一致的家族遗传树,cgMLST显示了略高的分辨率.

结论:

无论是cgMLST还是传统的MLST都适合用于M. hyosynoviae的流行病学分析.
cgMLST提供了增强的分辨率,用于区分密切相关的隔离物.
这些方法将有助于对M. hyosynoviae的科学和诊断调查.

关键词:

在CgMLST中使用.流行病学流行病学在MLST上,我们可以看到MLST.菌质体是低联的菌体猪猪的猪猪的猪猪的猪猪的猪

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