利科哈尔科恩A和B通过调节肌素调节来增强肌肉的增殖和分化
Khurshid Ahmad1, Eun Ju Lee1, Shahid Ali1
1Department of Medical Biotechnology, Yeungnam University, Gyeongsan 38541, South Korea; Research Institute of Cell Culture, Yeungnam University, Gyeongsan 38541, South Korea.
概括
草甘 (Glycyrrhiza uralensis) 提取物及其化合物Licochalcone A和B抑制肌素 (MSTN),促进肌肉生长,并可能对抗肌肉衰老. 这些天然化合物为肌肉缩障碍提供治疗选择.
科学领域:
- 自然产品化学 自然产品化学
- 分子生物学分子生物学
- 老年学是指老年学的学科.
背景情况:
- 肌静素 (MSTN) 抑制是一种有前途的治疗策略,用于肌肉损失条件.
- 草甘 (Glycyrrhiza uralensis) 粗水提取物 (CWE) 和其化合物液化基因因因,四氧化甲基和液化B (Licochalcone B) 之前已被证明可以抑制MSTN并促进肌肉形成.
- 特定的G. uralensis化合物加速肌肉再生,增强肌细胞增殖和分化.
研究的目的:
- 研究G. uralensis,Licochalcone A (Lic A) 和Lic B在通过抑制MSTN来调节肌肉质量的潜力.
- 评估Lic A和Lic B对衰老和肌肉形成过程的影响.
- 基于之前关于G. uralensis及其成分在肌肉健康方面的发现.
主要方法:
- 在G. uralensis化合物与MSTN及其受体ACVRIIB.的基分子对接和动态模拟中.
- 使用C2C12细胞系和初级小鼠肌肉干细胞进行体外研究,以评估增殖,分化和分子标记物 (MSTN,Atrogin 1,MuRF1).
- 在小鼠模型中的体内实验测量肌肉纤维直径并评估肌肉细胞的抗衰老作用.
主要成果:
- Lic A和Lic B与MSTN结合,减少其与ACVRIIB的相互作用,并抑制下游信号传输.
- 使用G. uralensis CWE,Lic A或Lic B降低了MSTN,Atrogin 1和MuRF1在体外和体内水平.
- 观察到肌肉纤维直径增加,增强C2C12细胞增殖和分化,以及对老化的细胞的抗衰老作用.
结论:
- G. uralensis CWE,Lic A和Lic B表现出作为天然MSTN抑制剂的潜力.
- 这些化合物表现出抗肌肉衰老的特性.
- 研究结果表明,肌肉缩障碍和与年龄相关的肌肉衰减具有治疗潜力.
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