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个体代谢专业化和多功能性之间的权衡决定了微生物群落的代谢效率

Miaoxiao Wang¹, Xiaoli Chen², Yuan Fang³

¹College of Engineering, Peking University, Beijing 100871, China; Department of Environmental Systems Science, ETH Zürich, Zürich 8092, Switzerland; Department of Environmental Microbiology, Eawag, Dübendorf 8600, Switzerland.

|January 18, 2024

概括

工程微生物联盟用于代谢分工 (MDOL) 可以增强功能. 这项研究发现,工程联盟的特定配置在甲烯降解方面优于单一菌株和复杂的MDOL系统.

科学领域:

合成生物学合成生物学
微生物生态学微生物生态学
代谢工程是代谢工程.

背景情况:

微生物中的代谢途径可以由单个种群进行或通过代谢分工 (MDOL) 分布.
MDOL提供了降低代谢负担等好处,但可以通过低效的中间交换来限制.
社区的功能取决于个体代谢专业化和多功能性之间的平衡.

研究的目的:

在微生物联盟中实验性地研究代谢专业化和多功能性之间的权衡.
在工程微生物系统中确定代谢分工 (MDOL) 的最佳配置.
设计具有增强降解能力的合成联盟.

主要方法:

将乙烯降解途径分解成四个不同的步骤.
设计不同的微生物菌株,具有不同的代谢专业化水平.
构建和评估了1456个合成微生物联盟,以提高纳烯降解效率.

主要成果:

确定了74个合成联盟,这些联盟超过了自治群体和严格的MDOL联盟的退化功能.
证明特定的MDOL配置可以克服中间交换效率的局限性.
定量建模为优化MDOL设计提供了策略.

结论:

代谢专业化和多功能性的平衡对于微生物社区的功能至关重要.
与自然或高度专业化的系统相比,工程合成联盟可以实现更高的性能.
这项研究提供了通过战略MDOL实施设计高性能微生物系统的见解.

关键词:

功能冗余性的功能冗余性代谢劳动分工的代谢分工.代谢专业化代谢专业化代谢的多功能性代谢的多功能性微生物群落中的微生物群落.

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