胚胎转移到使用克洛米芬酸盐和短效孕激素制备的无菌接收母马中
M K Derbala1, T E Mosallam1, R A Sindi2
1Animal Reproduction Research Institute (ARRI), Agriculture Research Center (ARC), 5 - Hadyek EL-Behoth St. Haram, PO: 12556, Giza, Egypt.
Journal of equine veterinary science
|January 18, 2024
概括
使用克洛米芬酸盐 (CC) 和Altrenogest的新协议有效地为胚胎移植准备了非循环受体母马. 与传统方法相比,这种马匹育种的进步导致了更高的怀孕率.
科学领域:
- 生殖生物学 生殖生物学
- 兽医医学 兽医医学 兽医医学
- 马科的科学 马科的科学
背景情况:
- 胚胎移植 (ET) 对于马匹育种计划中的遗传改进至关重要.
- 有效的接受母马准备对于成功的ET至关重要,但对非循环母马的协议具有挑战性.
- 目前准备受体母马的方法往往需要优化以提高效率.
研究的目的:
- 评估一种用于准备非循环受体母马进行胚胎移植的新方案.
- 评估克洛米芬酸盐 (CC) 和口服Altrenogest在同步非循环马匹中的疗效.
- 为了比较使用新方案治疗的母马与周期性对照对象的怀孕率.
主要方法:
- 72只本地品种母马被分为两组:60只非周期性母马 (第一组) 和12只周期性对照 (第二组).
- 第1组接受了口服克洛米芬酸盐 (CC) 4天,然后口服阿尔特雷诺杰斯特6天.
- 超声波监测了子宫回声质和怀孕,并评估了血清雌激醇和孕激素水平.
主要成果:
- 用CC和Altrenogest (1组) 治疗的母马的血清孕激素度明显高于对照组 (P < 0.001).
- 在期期间,雌激醇度在各组之间没有显著差异 (P > 0.05).
- 第1组与第2组相比 (66.6%) 实现了更高的怀孕率 (83.3%).
结论:
- 使用口服克洛米芬酸盐 (CC) 和Altrenogest的新协议对准备非循环受体母马有效.
- 该协议提供了一种可行的解决方案,用于提高非周期性母马的胚胎移植成功率.
- 这些发现支持使用CC和Altrenogest来增强马匹育种技术.
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