AP-2α/AP-2β转录因子是表皮平衡的关键调节者
Hui Zhang1, Jackelyn R Raymundo1, Kathleen E Daly1
1Cutaneous Biology Research Center, Department of Dermatology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, USA.
The Journal of investigative dermatology
|January 18, 2024
概括
AP-2转录因子对于成人皮肤健康至关重要. 皮肤细胞中AP-2α和AP-2β的损失导致分化缺陷,炎症和头发异常,影响表皮平衡.
科学领域:
- 分子生物学分子生物学
- 皮肤病学 皮肤病学
- 发育生物学是发展生物学.
背景情况:
- AP-2转录因子是已知的外皮发育的调节者.
- 它们在维持成年人皮肤平衡中的具体作用在很大程度上仍未被描述.
研究的目的:
- 为了研究AP-2α和AP-2β在成人表皮平衡中的功能.
- 确定不同水平的AP-2活性对皮肤结构和功能的影响.
主要方法:
- 在小鼠角质细胞中,AP-2α,AP-2β或两者的遗传失活化.
- 评估皮肤和头发的表型.
- 对受影响的表皮进行转录分析.
- 在成年小鼠中诱导性基因失活.
主要成果:
- AP-2α是成年表皮中占主导地位的AP-2因子,AP-2β也存在.
- AP-2β的损失由AP-2α补偿,但AP-2α缺乏会损害角质细胞分化和头发发育.
- 综合AP-2α/AP-2β损失导致严重的皮肤和头发缺陷,先于中性友好性炎症.
- 转录组学揭示了差异化受损,提升了警报基拉丁,以及改变了免疫调节剂.
- 由于AP-2α损失导致CXCL14和K15表达的减少与皮肤炎症有关.
结论:
- AP-2α和AP-2β对于维持成人皮肤上皮质平衡至关重要.
- 这些转录因子调节质细胞分化和头发形态发生.
- 失调AP-2因子通过改变基因表达,包括CXCL14和K15.的皮肤炎症,包括CXCL14和K15.
相关概念视频
Renewal of Skin Epidermal Stem Cells
2.5K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.5K
General Transcription Factors
5.3K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.3K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
Transcription Factors
75.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
75.9K
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
RNA Polymerase II Accessory Proteins
9.2K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.2K


