转录中的分子切换通过剪接和proline-isomerization调节了植物的压力反应
Frederik Friis Theisen1,2, Andreas Prestel2, Steffie Elkjær1
1The REPIN and The Linderstrøm-Lang Centre for Protein Science, Department of Biology, University of Copenhagen, Copenhagen, Denmark.
Nature communications
|January 18, 2024
概括
阿拉比多普西斯DREB2A使用双价结合开关来调节压力期间的基因表达. 这种切换涉及与转录协调器相互作用的独特动机,允许精确控制应激反应.
科学领域:
- 植物分子生物学 植物分子生物学
- 转录因子调节 转录因子调节
- 压力信号通道是压力信号通道.
背景情况:
- 在Arabidopsis thaliana中DREB2A转录因子集成压力信号.
- 它与RCD1和中间体复合体亚单元Med25的相互作用对基因表达至关重要.
- 这种相互作用的精确分子机制仍然不清楚.
研究的目的:
- 阐明DREB2A与RCD1和Med25相互作用的分子机制.
- 为了描述DREB2A中的双价结合开关.
- 了解替代拼接如何影响DREB2A功能.
主要方法:
- 生物物理分析分析
- 结构分析 (包括NMR)
- 高通量选的高通量选
- 对DREB2A拼接变体的分析.
主要成果:
- 在DREB2A中发现了一种双价结合开关,该开关具有一种ACID结合动机 (ABS) 和一种RCD1结合动机 (RIM).
- 一种缺乏RIM的压力诱导的拼接变体保留了转录活性.
- ABS和RIM结合到Med25-ACID上不同的位点,形成一个结构异质的复合体,受素 cis/trans异构化的影响.
- 异构化会影响二次结构的稳定性,并促进激活剂/抑制剂的交换.
结论:
- 对于转录调节,DREB2A采用了一个转录后和翻译后开关.
- 这种开关允许压力信号的动态集成.
- 替代拼接和蛋白质异构化有助于微调植物应激反应.
相关概念视频
Chromatin Structure Regulates pre-mRNA Processing
7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
7.0K
Transcription
147.0K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
147.0K
Alternative RNA Splicing
21.2K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.2K
Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
Responses to Heat and Cold Stress
13.5K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.5K
RNA Splicing
56.4K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.4K


