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构建近红外光系统,用于对生物组织进行成像

Xu Zhao^1,2, Shilin Li^1,2, Yue Song³

¹Department of Urology, General Hospital of Northern Theater Command, Shenhe District, No. 83, Wenhua Rd, Shenyang, 110000, China.

Scientific reports

|January 18, 2024

概括

这项研究开发了一种具有成本效益的光成像设备,使用印ocyanine Green (ICG) 来监测手术期间的膀变化. 该系统通过跟踪ICG光强度来有效地可视化器官和指导程序.

科学领域:

生物医学工程生物医学工程
手术技术手术技术
医疗成像医学成像

背景情况:

手术精度往往受到依赖无助视觉或昂贵的内镜工具的限制.
实时手术内成像可以改善重要组织的识别,分离和保护.
高昂的设备成本阻碍了先进的外科可视化技术的采用.

研究的目的:

优化和开发一个成本效益高的光成像设备,利用印ocyanine绿色 (ICG).
为了监测膀内的ICG的平均光强度变化,以提供外科指导.
为涉及膀和其他空心器官的手术提供见解.

主要方法:

开发了器官和组织的模块化光成像系统.
在雄性子中,通过盆和耳静脉给药ICG (0.01毫克/毫升),用于尿道,膀和小肠成像.
在子中用ICG进行逆行性膀输液,以捕获依赖时间的光图像.
使用Image Pro Plus 6.0和Prism 8.0进行曲线生成的量化平均光强度.

主要成果:

开发的设备成功地在动物模型中成像了尿管,膀和小肠.
膀ICG光强度显示了随着时间的推移的动态变化,与尿液产生和尿管结合相关.
在结合与非结合尿道条件下观察到明显的光强度模式.

结论:

一个模块化,经济高效的器官和组织光成像系统已经成功开发和优化.
该系统有效用于动物模型中空心器官的成像,为手术程序提供潜在的好处.
这项技术通过监测目标器官的光变化,提供了宝贵的手术内指导.

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Total Internal Reflection Fluorescence Microscopy

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