使用乳制品加工废物生产,优化,扩大规模和表征聚二甲酸共聚合物
Tejaswini Dhanaji Patil1, Saptaneel Ghosh1, Aparna Agarwal2
1Department of Dairy Science and Food Technology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India.
Scientific reports
|January 18, 2024
概括
乳制品废弃物 (乳清) 可以使用Ralstonia eutropha.转化为可生物降解的多基酸盐 (PHA) 生物聚合物. 优化氨基酸和tween-80度显著提高了PHA生产,为塑料提供了一个可持续的替代品.
科学领域:
- 生物技术是生物技术.
- 环境科学 环境科学
- 聚合物科学 聚合物科学
背景情况:
- 石化塑料是不可降解的,这给环境带来了挑战.
- 微生物生物转化提供了一种可持续的生产可生物降解聚合物的途径.
- 乳制品行业的废物,如乳清,为低成本原料提供了机会.
研究的目的:
- 调查脱蛋白乳清用于聚酸酸 (PHA) kopolimer生产的使用.
- 使用Ralstonia eutropha优化PHA生产,并确定关键的过程变量.
- 评估从乳制品废物中提升PHA生产的可行性.
主要方法:
- 用于PHA生产的微生物菌株 (Ralstonia eutropha和Bacillus megaterium) 的查.
- 优化工艺变量 (氨基酸和tween-80度) 使用中心复合可旋转设计.
- 使用ESI-MS,FTIR和TEM进行产生的PHA的表征.
- 将PHA生产升级到3.0L生物反应器.
主要成果:
- 与Bacillus megaterium相比,Ralstonia eutropha表现出更高的PHA产量.
- 在优化条件下 (托芬50毫克L-1和tween-80 3毫升L-1),聚乙酸 (PHB) 的质量分数为69.3%,超过预测的62.8%.
- 获得了增强的R.安抚体质量增长 (6.80 g L-1) 和PHB含量 (4.71 g L-1).
- 在3.0L生物反应器中进行大规模生产,保持了类似的PHA含量和产量.
结论:
- 脱蛋白乳清是生产高含量生物聚合物 (PHA) 的可行且廉价原料.
- 使用Ralstonia eutropha的微生物生物转化为废物管理和生物聚合物生产提供了一种可持续的方法.
- 优化的生物工艺可以有效地将乳制品废物转化为有价值的可生物降解材料.
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