Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Spatial imprints of emergent cardiomyocyte states in the pressure-overloaded heart.

bioRxiv : the preprint server for biology·2026
Same author

Photosynthesis regulation impacts carbon and nitrogen assimilation in the diazotrophic cyanobacterium Anabaena sp. PCC 7120.

The New phytologist·2026
Same author

Cluster assembly dynamics drive fidelity of planar cell polarity polarization.

Nature communications·2026
Same author

The COX2-PGE<sub>2</sub>-PKA Axis Suppresses Antiviral Immunity by Inhibiting mtDNA-Dependent STING Activation.

bioRxiv : the preprint server for biology·2026
Same author

Engineering the mechanosensitivity of single DNA molecules via high-throughput microfluidic force spectroscopy.

bioRxiv : the preprint server for biology·2026
Same author

Better reporting is better science: Community-defined minimal reporting requirements for light microscopy.

The Journal of cell biology·2026
Same journal

ClairS: a deep-learning method for long-read tumor-normal pair somatic small variant calling.

Nature methods·2026
Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026
Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026
Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026
Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026
Same journal

Deep molecular profiling in three dimensions.

Nature methods·2026
查看所有相关文章

相关实验视频

Updated: Jul 5, 2025

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

8.5K

内容感知框架插值 (CAFI):基于深度学习的时间超分辨率,用于快速生物成像.

Martin Priessner1,2, David C A Gaboriau3, Arlo Sheridan4

  • 1Department of Chemistry, Imperial College London, London, UK. martin.priessner@gmail.com.

Nature methods
|January 18, 2024
PubMed
概括
此摘要是机器生成的。

内容感知插入 (CAFI) 使用深度学习来预测显微镜视频中缺失的. 这增强了时间分辨率,使得能够更好地观察快速的细胞动态,减少光毒性.

更多相关视频

Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations
13:13

Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations

Published on: March 19, 2021

2.9K
Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

4.8K

相关实验视频

Last Updated: Jul 5, 2025

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

8.5K
Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations
13:13

Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations

Published on: March 19, 2021

2.9K
Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

4.8K

科学领域:

  • 生物物理学的生物物理.
  • 细胞生物学 细胞生物学
  • 显微镜的使用方法

背景情况:

  • 高分辨率显微镜可实现细胞过程的3D和时差成像.
  • 观察快速的细胞动态受光漂白和光毒性限制.

研究的目的:

  • 实施和评估基于深度学习的内容意识框架插值 (CAFI) 网络,以改善显微镜中的时间分辨率.
  • 评估CAFI准确预测中间的能力,并了解生物运动背景.

主要方法:

  • 实施两种CAFI深度学习网络:放大SlowMo和深度感知视频插值.
  • 来自四种显微镜模式的12个不同数据集的CAFI性能比较.
  • 应用CAFI用于单粒子跟踪和核细分.

主要成果:

  • CAFI准确地预测中间图像,增强图像系列的时间分辨率.
  • 与标准插值方法相比,CAFI通过理解运动上下文来表现出优越的性能.
  • 验证了CAFI在单粒子跟踪和核细分任务中的实用性.

结论:

  • CAFI显著提高了显微镜成像中的时间分辨率,促进了快速细胞动态的研究.
  • CAFI提供了一种潜在的解决方案,可以减少光照和光毒性,从而改善长期活细胞成像.
  • 开发的CAFI模型和数据可以通过ZeroCostDL4Mic平台访问,以获得更广泛的研究应用.