评估单个植入前胚胎的组织组织蛋白修饰分析
Yiren Zeng1, Yoichiro Hoshino1, Kazuki Susami1
1Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
BMC genomics
|January 18, 2024
概括
新的Well-of-the-Well CUT&Tag (WOW-CAT) 方法简化了单个植入前胚胎中的全基因组组组织蛋白修饰分析. 这种技术提高了植入前表观遗传诊断的效率和准确性.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 发展生物学 发展生物学
- 基因组学就是基因组学.
背景情况:
- 之前的工作建立了NTU-CAT,用于在单个植入前胚胎中进行全基因组基因组基因组修饰分析.
- 目前的研究简化了NTU-CAT使用Well-of-the-Well (WOW) 系统,创建了WOW-CUT&Tag (WOW-CAT).
研究的目的:
- 为了简化和提高单个胚胎基因组修饰分析的效率.
- 为了实现灵活的表观遗传分析,用于植入前遗传诊断.
主要方法:
- 使用了Well-of-the-Well (WOW) 系统,同时处理多个胚胎.
- 应用WOW-CUT&Tag (WOW-CAT) 用于从单个胚芽细胞或其部分中进行全基因组基因组修饰概况.
- 在同一胚胎中同时对两个基因组修饰 (H3K4me3和H3K27ac) 进行了分析.
主要成果:
- WOW-CAT 启用了从单个胚胎囊甚至部分胚胎囊中基因组修饰概况.
- WOW-CAT 产生了与 NTU-CAT 相似的 H3K4me3 配置文件,但更接近 ChIP 测序标准,错误阳性较少.
- 在同一胚胎的两半中同时分析H3K4me3和H3K27ac.
- 成功地将WOW-CAT应用于活检的肌皮细胞,用于植入前的表观遗传诊断,包括通过XIST和DDX3Y的H3K4me3分析进行胚胎性别测定,而无需深度测序.
结论:
- WOW-CAT为单个胚胎的表观遗传学分析提供了一种简化,高效和灵活的方法.
- 该技术适用于植入前表观遗传诊断,包括非侵入性的胚胎性别.
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