相关实验视频

Updated: Jul 5, 2025

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

Published on: September 12, 2019

依赖MTFR2的线粒体裂变促进了HCC的进展

La Zhang¹, Xiuzhen Zhang², Haichuan Liu¹

¹Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.

Journal of translational medicine

|January 18, 2024

概括

这项研究确定了与肝细胞癌 (HCC) 中的线粒体裂变相关的十个基因签名. 这个签名预测了患者的预后,并突出了MTFR2作为HCC的潜在治疗标.

科学领域:

线粒体生物学线粒体生物学
在瘤学瘤学.
基因组学就是基因组学.

背景情况:

线粒体动力学 (裂变,融合,线粒体衰变) 在癌症中至关重要,但它们在肝细胞癌 (HCC) 中的特殊作用需要进一步调查.
了解HCC中的线粒体动力学可以揭示新的预后标志物和治疗点.

研究的目的:

研究线粒体动态对HCC进展的影响.
为HCC患者开发基于线粒体动态基因的预后模型.
探索线粒体状态,免疫透和HCC治疗反应之间的关系.

主要方法:

分析了来自MitoCarta 3.0的线粒体动态基因和TCGA的基因表达数据.
根据基因表达,患者被分组成群,并使用COX和LASSO回归构建了一个预后模型.
评估了免疫透,瘤突变负担 (TMB),瘤干度指数 (TSI) 和免疫检查点阻断 (ICB) 反应.
使用TEM,H&E,IHC,WB和IF进行了形态和蛋白质表达分析.

主要成果:

确定了两个患者集群,其中集群2显示线粒体裂变增加和预后更差.
开发了一种十基因的预后特征,所有基因都在高风险组上调节,并与MTFR2介导的线粒体裂变有关.
高风险患者表现出不良的预后,较高的TP53突变频率,增加的免疫检查点表达,较高的TIS和较低的TIDE得分.
在HCC组织和细胞系中证实了线粒体分裂的增加和MTFR2和DNM1L表达的升高.

结论:

一种与MTFR2和线粒体分裂相关的新型十基因预后特征被开发用于HCC.
这些基因对线粒体裂变至关重要,并显示出作为HCC的预测生物标志物和治疗点的潜力.

关键词:

核裂变是一种裂变.肝细胞癌是肝细胞癌.在MTFR2中,我们使用了MTFR2.线粒体动力学的动态预测模型是一个预测模型.

更多相关视频

A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma

A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma

Published on: September 30, 2021

Non-Invasive PET/MR Imaging in an Orthotopic Mouse Model of Hepatocellular Carcinoma

Non-Invasive PET/MR Imaging in an Orthotopic Mouse Model of Hepatocellular Carcinoma

Published on: August 31, 2022

相关概念视频

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...