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与卵泡液质量相关的microRNAs会影响卵细胞和早期胚胎发育

Sogo Aoki¹, Yuki Inoue¹, Shunsuke Hara¹

¹Department of Animal Science, Graduate School of Agriculture Tokyo University of Agriculture Atsugi City Kanagawa Japan.

Reproductive medicine and biology

|January 19, 2024

概括

毛囊液微RNAs (miRNAs) 是卵细胞和胚胎发育的关键. 在体外成熟介质中补充特定的miRNAs,miR-151-3p和miR-425-5p,可以增强胚胎发育和卵细胞成熟.

科学领域:

生殖生物学生殖生物学
分子生物学分子生物学
动物科学动物科学

背景情况:

卵细胞和胚胎质量在个体之间有很大差异.
毛囊液 (FF) 对于卵细胞成熟至关重要,并可能影响卵巢环境.
在FF内的细胞外囊泡支持卵细胞成熟和胚胎发育,当添加到体外成熟 (IVM) 和培养 (IVC) 介质时.

研究的目的:

调查FF中微RNA (miRNA) 配置文件对卵细胞成熟和胚胎发育至关重要的假设.
确定与优质FF和增强胚胎发育相关的特定miRNA.

主要方法:

从奶牛中收集了毛囊液 (FFs),并根据封闭的卵细胞的发育速度将其分类为"好"或"差".
小RNA测序被用来识别与Good FFs相关的miRNAs.
将FF和已识别的miRNA添加到IVM或IVC介质中,以评估它们对卵细胞成熟和胚胎发育的影响.

主要成果:

补充Good FF可以改善胚胎发育.
良好的FF显示了miR-151-3p和miR-425-5p的较高度,相比于差的FF.
补充miR-151-3p或miR-425-5p可以单独增强胚胎发育到囊胚阶段.

结论:

在FF中的微RNA配置文件与卵细胞成熟和胚胎发育有显著的关联.
特定的miRNAs,miR-151-3p和miR-425-5p,可以用于改善体外卵细胞成熟和胚胎发育.

关键词:

牛肉类的牛肉类的牛肉类.早期胚胎发育的早期发展.细胞外囊泡细胞外囊泡毛囊液体毛囊液体毛囊液体这是一个小RNARNA.

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