神经原始细胞相关的转录因子FoxG1调节心脏上心脏细胞的增殖
Lucy Pilcher1,2, Lara Solomon1,2, Julie A Dragon3
1Department of Medicine, Cardiovascular Research Institute, University of Vermont, Colchester, VT 05446, USA.
Stem cells international
|January 19, 2024
概括
跑步运动通过影响心表细胞来促进有益的心脏生长. 转录因子FoxG1和非编码RNA Snord116是表心细胞增殖的关键调节者,可能会影响心脏重塑.
科学领域:
- 心血管生物学 心血管生物学
- 发展生物学 发展生物学
- 运动生理学 运动生理学
背景情况:
- 副心脏是心脏表面的一层介质细胞,通过表皮转介质转移 (EMT) 在心脏发育和修复中起着至关重要的作用.
- 由有氧运动诱导的生理性心脏缩与心脏功能和血管生成的改善有关,但心表细胞在这一过程中的作用尚不清楚.
研究的目的:
- 通过跑步运动诱导的生理性心脏缩期间调查本地表心细胞的功能.
- 确定运动影响心上细胞行为的分子机制.
主要方法:
- 通过酶消化和磁激活细胞分类 (MACS) 来分离来自跑步训练和对照小鼠的本地表心细胞.
- 通过cDNA微阵列测定,分析心上细胞的基因表达变化.
- 在培养后心细胞中使用shRNA介导的淘汰对FoxG1的功能性评估.
主要成果:
- 跑步运动增加了小鼠心表细胞中FoxG1 (一种转录因子) 和Snord116 (一种小型非编码RNA) 的转录.
- 在培养后心脏细胞中,FoxG1的淘汰显著降低了细胞增殖和Snord116的表达.
结论:
- FoxG1是表心细胞增殖的关键调节者.
- 这些发现表明,运动诱导的表心细胞变化,由FoxG1和Snord116介导,可能有助于有益的心脏重塑和改善心脏功能.
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