对小麦 (Triticum aestivum L.) 育种材料在体外雄性生殖潜力的研究
N V Petrash1, T N Kapko1, V V Sovetov1
1Siberian Research Institute of Plant Production and Breeding - Branch of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Vavilovskii zhurnal genetiki i selektsii
|January 19, 2024
概括
双倍平分体技术通过产生 homozygous 线来加速小麦的育种. 这项研究确定了特定的小麦品种和杂交品种,它们具有高响应性,能够有效地进行体外雄性生殖和新品种开发.
科学领域:
- 植物育种和生物技术
- 农业科学 农业科学
- 遗传学和基因组学 遗传学和基因组学
背景情况:
- 双倍平分体 (DH) 技术对于植物育种中同卵性菌株的快速发展至关重要.
- 评估体外雄性生殖是识别精英生殖质的关键,用于加速繁殖计划.
- 西伯利亚植物生产和育种研究所 (SibRIPP) 利用有前途的植物材料改进小麦.
研究的目的:
- 评估小麦初始育种材料的另一种培养中的体外雄性生殖指标.
- 为了识别小麦加入和杂交组合与卓越响应雄激素的诱导.
- 评估DH技术在创造新的小麦品种方面的潜力.
主要方法:
- 培养被用来诱导十种小麦品种和九种F1/F2杂交组合的雄性发育.
- 在Chu (N6) 培养基上培养植物材料,补充了1mg/l的2,4-D.
- 评估的关键参数包括新形成,白色,绿色和总再生植物.
主要成果:
- 观察到显著的基因型影响 (p < 0.01) 对雄性生殖特征.
- 诺沃西比里斯卡亚15 × 卢特森ShT-335的F2混合体显示了最多的新形成.
- 新西伯利亚15 × 卢特森ShT-335的F1杂交植物产生了最多的绿色植物,这表明再生潜力很高.
结论:
- 诺沃西比里斯卡亚15号表现出极好的响应能力,并被推作为高混合动力雄性生殖的家长线.
- 特定的F1和F2杂交物表现出显著的异质效应对雄性生殖,有价值的繁殖.
- DH技术有效地使得从选定的繁殖材料中快速创建DH系.
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