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Circ-ZNF236通过调节LGR4诱导的自细胞调节,通过调节角乳头分化的干细胞进行调解

Ya Xiao^1,2, Luyao Chen^1,2, Yunlong Xu³

¹Key Laboratory of Oral Diseases of Jiangsu Province and Stomatological Institute of Nanjing Medical University, Nanjing, China.

International endodontic journal

|January 19, 2024

概括

这项研究确定circ-ZNF236是从上皮囊 (SCAPs) odonto/osteogenic分化的干细胞的关键调节者. 通过miR-218-5p/LGR4通路激活自,Circ-ZNF236促进骨的形成.

科学领域:

再生医学是一种再生医学.
干细胞生物学干细胞生物学
分子生物学分子生物学

背景情况:

上皮皮的干细胞 (SCAPs) 对于牙和骨组织再生至关重要.
循环RNAs (circRNAs) 在干细胞分化中起着重要作用,包括骨质生成.
在SCAP odonto/osteogenic分化中circRNAs的特定机制仍未得到充分探索.

研究的目的:

为了识别和描述参与SCAP odonto/osteogenic分化中的新型circRNAs.
阐明Circ-ZNF236在SCAP分化中的功能背后的分子机制.
研究circ-ZNF236在骨再生中的治疗潜力.

主要方法:

使用桑格序列和原始放大方法识别和结构确认circ-ZNF236.
通过CCK-8,流细胞计和EDU测定评估SCAP增殖.
使用qRT-PCR,ALP活性和阿里扎林红色S染色对odonto/骨质性分化进行评估.
通过使用FISH,双露西法酶试验和西式涂抹,对circ-ZNF236/miR-218-5p/LGR4轴的研究.
使用TEM和mRFP-GFP-LC3 lentivirus检测自激活.
在老鼠头骨缺陷模型中进行体内骨形成评估.

主要成果:

一个稳定的circRNACirc-ZNF236在SCAP分化过程中被上调,并且在不影响增殖的情况下被正调 odonto/osteogenic分化.
Circ-ZNF236作为miR-218-5p的海绵,它针对LGR4mRNA.
通过调节miR-218-5p/LGR4通路并激活自,Circ-ZNF236促进了SCAP分化.
在大鼠头骨缺陷模型中,circ-ZNF236增强骨形成的过度表达.

结论:

Circ-ZNF236通过增加LGR4表达来激活自,从而增强SCAP的骨/骨质分化和骨形成.
在再生医学中,Circ-ZNF236是促进SCAP分化的一个有希望的治疗标.

关键词:

这是一个微型RNA.自自是自的过程.圆形的RNARNA是一个圆形的RNA.不同化的差异化差异化.来自顶端乳头的干细胞.

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