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通过声泡细胞相互作用通过血脑屏障增强的宏分子物质扩散

Jifan Chen¹, Jean-Michel Escoffre², Oliver Romito³

¹Department of Ultrasound in Medicine, The Second Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang University, Zhejiang, China; Inserm UMR 1253, iBrain, Université de Tours, Inserm, Tours, France.

Ultrasonics sonochemistry

|January 19, 2024

概括

超声波和微泡技术通过细胞膜变形打开血脑屏障 (BBB),促进药物输送的转细胞形成. 这个过程涉及信号传递和蛋白激酶C通路,增强宏分子的透性.

科学领域:

神经科学是一个神经科学.
生物医学工程生物医学工程
药理学药理学是指药理学的学科.

背景情况:

血脑屏障 (BBB) 对于维持大脑平衡和保护神经组织至关重要.
超声波 (US) 和微泡 (MB) 介导的BBB打开是一种有前途的药物输送技术,但其精确的机制尚不清楚.

研究的目的:

通过实验室模型研究细胞转移在血脑屏障暂时开放中的作用.
为了阐明底层的细胞和分子机制美国/MB介导的BBB透性.

主要方法:

在体外血脑屏障模型.
(Ca2+) 影像成像. (Ca2+) 的成像.
电子显微镜. 电子显微镜.
电生理学记录. 电生理学记录.
对500kDa光德克斯运输的评估.

主要成果:

美国 + MBs诱导了显著的内皮细胞膜变形.
通过激素,洞穴和克拉特林介导的途径促进大分子 (500 kDa 德克斯) 的转细胞形成.
细胞溶液Ca2+度的增加与增强的德克斯透度相关.
观察到与Ca2+-蛋白激酶C (PKC) 信号通路的相关性.

结论:

声学介导的微泡-内皮细胞相互作用触发了膜变形和转细胞.
信号在US/MB诱导的BBB透性中起着关键作用.
这项研究提供了对整个BBB的宏分子药物递送机制的见解.

关键词:

血液-大脑屏障是什么?气泡细胞机械相互作用药物交付是药物交付的过程.在体外试验中宏分子物质是一种宏分子物质.微气泡是一种微气泡.转细胞化是通过细胞的.超声波超声波是指超声波的使用.

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