鲁布斯黄网病毒在树基因组中的整合:一个几个世纪以来的故事
Thien Ho1, Janet C Broome1, Jason P Buhler1
1Driscoll's Inc., Watsonville, CA, 95076, USA.
Virology
|January 19, 2024
概括
鲁布斯黄网病毒 (RYNV) 在树中以内源序列的形式广泛存在. 繁殖努力集中在有限的胚胎质上,导致这些非功能性病毒残留物的流行.
科学领域:
- 植物病毒学 植物病毒学
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
背景情况:
- 黄网病毒 (RYNV) 是一种属于Badnavirus属的植物病毒.
- 恶性病毒可以存在于植物基因组内作为内源性,不活跃的序列或作为传染性插曲性形式.
- 了解RYNV在Rubus生殖质中的地位对于植物卫生和育种计划至关重要.
研究的目的:
- 研究各种Rubus品种内源RYNV (endoRYNV) 序列的存在和多样性.
- 开发基因型测定试验,以表征不同的EndRYNV血统和病例性RYNV.
- 评估endoRYNV存在于商业低生殖质中的影响.
主要方法:
- 多种拉斯伯里品种的全基因组测序.
- 对测序基因组和公共拉斯伯里基因组数据集的生物信息分析.
- 开发和应用用于内分泌RYNV和病例性RYNV基因型定型的分子测试.
主要成果:
- 在Rubus生殖质中识别了各种各样的内源RYNV序列.
- 从几乎完整的非功能性基因组到短片段的endoRYNV序列的表征.
- 证实了在商业大树中广泛存在的endoRYNVs,与有限的生殖质池有关.
结论:
- 内源的RYNV序列在商业拉斯伯里品种中普遍存在.
- 草育种中对一个狭窄的遗传基础的关注很可能放大了endoRYNVs的存在.
- 需要进一步研究Rubus体内内RYNVs的影响和管理.
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