相关实验视频

Updated: Jul 5, 2025

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 17, 2010

在Marsupenaeus japonicus中对cathepsin C的表征和功能分析

Zuhao Tu¹, Jian Zhong², Hongquan Li²

¹Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang, China.

Fish & shellfish immunology

|January 19, 2024

概括

甲素C在的免疫力中起着至关重要的作用. 在Marsupenaeus japonicus中击败这个基因显著降低了对Vibrio alginolyticus和WSSV的抗病能力,突出了它在天生的免疫力中的重要性.

科学领域:

水产养殖是水产养殖的一种方式.
海洋生物学海洋生物学
免疫学免疫学免疫学

背景情况:

甲素C是一种关键的囊蛋白酶,参与蛋白质分解和免疫细胞蛋白酶激活.
了解它对病耐药性的作用对于水产养殖健康至关重要.

研究的目的:

为了研究 cathepsin C 在 Marsupenaeus japonicus 的天生的免疫力中的作用.
分析MjcathC基因的表达模式和功能意义.

主要方法:

来自Marsupenaeus japonicus. 的cathepsin C基因 (MjcathC) 的克隆.
在各种组织中对MjcathC表达的分析.
通过RNA干扰 (RNAi) 来抑制MjcathC表达.
挑战性带有Vibrio alginolyticus和WSSV. 这两种病毒.

主要成果:

鉴定了MjcathC基因序列,与其他penaeid物种具有很高的相似性.
MjcathC表达在肝脏中最高,并在病原体挑战时升高调节.
MjcathC的淘汰导致免疫相关基因的表达减少,挑战后死亡率增加.

结论:

甲素C对于Marsupenaeus japonicus的抗菌和抗病毒天生的免疫力至关重要.
MjcathC在病耐药性方面发挥着重要作用.
向甲素C可能是增强病耐药性的潜在策略.

关键词:

卡特赫普辛C C的时间表达式分析表达式分析基因克隆就是基因克隆.火星鱼 (Marsupenaeus japonicus) 是一个日本鱼.

更多相关视频

Purification of the Sarco-Endoplasmic Reticulum Ca2+-ATPase from Rabbit Muscle

Purification of the Sarco-Endoplasmic Reticulum Ca²⁺-ATPase from Rabbit Muscle

Published on: March 21, 2025

Microfocus X-ray CT microCT Imaging of Actinia equina Cnidaria, Harmothoe sp. Annelida, and Xenoturbella japonica Xenacoelomorpha

Microfocus X-ray CT microCT Imaging of Actinia equina Cnidaria, Harmothoe sp. Annelida, and Xenoturbella japonica Xenacoelomorpha

Published on: August 6, 2019

相关概念视频

Catenins

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...

Caspases

Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...