血管新生是由缺氧宫癌衍生的细胞外囊泡促进的,这取决于内皮细胞环境
E Orozco-García1, V Getova2, J C Calderón3
1Physiology and Biochemistry Research Group - PHYSIS, Faculty of Medicine, University of Antioquia, Medellin, Colombia; University of Groningen, University Medical Center Groningen, Department of Pathology and Medical Biology, Hanzeplein 1 (EA11), Groningen 9713 GZ, the Netherlands; University of Groningen, University Medical Center Groningen, W.J. Kolff Research Institute, Groningen, the Netherlands.
Vascular pharmacology
|January 19, 2024
概括
缺氧显著增加了癌细胞衍生的细胞外囊泡 (CA-EVs) 的产生和蛋白质含量. 这些CA-EVs促进血管生成,其作用由瘤微环境调节,影响癌症生长和转移.
科学领域:
- 癌症生物学 癌症生物学
- 瘤微环境研究 研究
- 细胞外囊泡生物学 细胞外囊泡生物学
背景情况:
- 癌症的进展依赖于血管生成,这是一个受瘤微环境 (TME) 影响的过程.
- 癌细胞衍生的细胞外囊泡 (CA-EVs) 参与调节TME并可能促进血管生成.
- 了解TME条件如何影响CA-EVs的血管生成潜力,对于解释瘤血管化和转移至关重要.
研究的目的:
- 研究瘤状况如缺氧如何影响CA-EVs的血管生成潜力.
- 为了确定目标细胞条件 (营养缺乏,细胞外基质) 是否影响CA-EVs的血管生成能力.
- 阐明CA-EVs在调节瘤血管化的作用及其对癌症的影响.
主要方法:
- 从Normoxia和 hypoxia下的HeLa和SiHa宫癌细胞系中分离和表征CA-EVs.
- 在体外使用内皮细胞 (EC) 和大动脉环测定评估CA-EVs的血管生成潜力.
- 在基底或完整的介质中培养EC和大动脉环,以模仿不同的细胞环境.
主要成果:
- 低氧增加了CA-EV的产量10-100倍和蛋白质含量2-4倍相比normoxic条件下.
- 在基底介质中培养的EC和大动脉环中,CA-EV治疗增强了管道形成和发芽.
- 在完整的培养基中培养的EC中,CA-EV稳定了已建立的血管网络.
结论:
- 缺氧会根据细胞系对CA-EV产生差异性影响,影响其血管性质.
- 细胞环境,包括营养的可用性和细胞外基质,显著影响CA-EVs如何影响内皮细胞血管生成潜力.
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