与H3K7me3甲基化相关的长非编码RNA在应激下负面调节OsZIP16转录
He Li1, Xue Song Liu2, Di Sun1
1Department of Biochemistry and Molecular Biology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Gene
|January 19, 2024
概括
米中的一种新型长非编码RNA (lncRNA),LncRNA-OsZIP16 (L16),调节 (Cd) 的积累. L16抑制OsZIP16金属转运基因,通过表观遗传机制帮助大米适应Cd压力.
科学领域:
- 植物分子生物学 植物分子生物学
- 环境毒理学环境毒理学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- (Cd) 是一种有毒的污染物,影响作物和人类健康.
- 了解农作物中Cd积累的遗传调节至关重要.
- 长非编码RNAs (lncRNAs) 在应激反应中的作用是一个新兴的领域.
研究的目的:
- 确定米中Cd积累的新型遗传调节剂.
- 阐明一个特定的lncRNA,LncRNA-OsZIP16 (L16) 和它的目标基因,OsZIP16.16的功能.
- 研究米中Cd应激适应的表观遗传机制.
主要方法:
- 在大米中的lncRNA TCONS_00502780 (L16) 和其目标金属载体ZIP16 (OsZIP16) 的功能识别.
- 通过CRISPR-Cas9基因编辑 (C16) 来淘汰OsZIP16.
- RNA干扰 (L16-R) 和过度表达 (L16-OE) 来操纵L16水平.
- 染色体免疫沉 (ChIP) 用于分析基因素甲基化标记 (H3K27me3).
主要成果:
- 在Cd压力下,L16抑制了OsZIP16的转录;L16是下调的,而OsZIP16是上调的.
- OsZIP16的淘汰 (C16) 增加了Cd的敏感性; OsZIP16的过度表达 (OE16) 减少了Cd的转位.
- L16敲击 (L16-R) 模仿OE16表型;L16过度表达 (L16-OE) 模仿C16表型.
- Cd压力和L16操纵改变了OsZIP16基因周围的H3K27me3标记.
结论:
- LncRNA-OsZIP16 (L16) 通过表观遗传修饰 (H3K27me3) 负面调节OsZIP16的表达.
- 这种L16-OsZIP16调节途径对于适应应激是必不可少的.
- 这些发现提供了关于植物应激反应中IncRNA介导的表观遗传调节的见解.
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