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人类托芬氧酶2的工程,以高效合成5-基托芬

BingBing Wang¹, Jian-Zhong Xu¹, Shuai Liu¹

¹The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800# Lihu Road, WuXi 214122, People's Republic of China.

International journal of biological macromolecules

|January 19, 2024

概括

研究人员设计了一种高活性型酸酶2 (TPH2) 突变体MT10,用于高效的5-基型 (5-HTP) 合成. 在单系统中,MT10实现了89%的转化率,大大改善了L-三胺的生产.

科学领域:

生物催化剂是一种生物催化剂.
酵素工程是什么? 酶工程是什么
生物技术是生物技术.

背景情况:

托芬氧化酶 (TPH) 催化L-托芬氧化以产生5-托芬 (5-HTP).
野生类型的人类TPH2表现出有限的活性,阻碍有效的5-HTP合成.

研究的目的:

提高人类TPH2的催化效率,以改善5-HTP的生产.
开发有效的生物转化系统来合成5-HTP.

主要方法:

半理性活性位点和测试 (CAST) 用于设计一种高活性TPH2突变体 (MT10).
开发了两个生物转化系统:一个体外单系统和一个全细胞催化系统 (WCCS).
用分子动力学模拟来分析酶基质相互作用.

主要成果:

与野生类型的TPH2.2相比,MT10突变的kcat/Km增加了2.85倍.
在一系统中,MT10在8小时内实现了89%的L-托芬转化为5-HTP的转化率.
使用MT10的单系统的性能优于M1变体,并且实现了比WCCS更高的转换率.

结论:

改造的MT10突变物显著提高了5-HTP生产的TPH2催化效率.
在体外单系统为工业5-HTP合成提供了有效和高效的方法.
在MT10中增强的酶基质相互作用有助于改善系统稳定性和性能.

关键词:

5 - - 氧三甲是一种5 - - 氧三甲.分子动力学模拟模型半理性的组合性活性位点和测试.托芬氧化酶是什么?

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