急性运动增加GDF15和未折叠的蛋白质反应/整合的应激反应在肌肉中的2型糖尿病
Rugivan Sabaratnam1,2, Jonas M Kristensen1,2,3, Andreas J T Pedersen2
1Department of Clinical Research & Department of Molecular Medicine, University of Southern Denmark, DK-5230 Odense M, Denmark.
The Journal of clinical endocrinology and metabolism
|January 19, 2024
概括
运动有效调节2型糖尿病 (T2D) 患者的生长分化因子15 (GDF15) 和展开蛋白质反应 (UPR) /综合应激反应 (ISR) 标志物. 这些代谢途径在急性炼和恢复期间不会受到T2D的影响.
科学领域:
- 代谢健康 代谢健康
- 运动生理学 运动生理学
- 分子生物学分子生物学
背景情况:
- 定期运动对于预防肥胖和2型糖尿病 (T2D) 至关重要.
- 运动诱导的分泌因子 (exerkines) 可能改善新陈代谢,而受损的反应可能导致心脏代谢疾病.
研究的目的:
- 为了调查运动诱导的增长分化因子15 (GDF15) 和其上游调节器的未展开的蛋白质反应 (UPR) /综合应激反应 (ISR) 的调节是否在T2D患者的骨肌肉中受损.
- 与体重匹配,耐葡萄糖个体进行比较.
主要方法:
- 13名男性T2D患者和14名体重匹配对照在70%的VO2max时进行了1小时的运动.
- 骨肌肉活检和血液样本是在运动前,运动后立即和恢复后3小时收集的.
- 分析了血清和肌肉GDF15水平,UPR/ISR标记物 (基因表达,蛋白质/酸化水平).
主要成果:
- 在两组中,急性运动增加了肌肉GDF15表达和血清GDF15水平.
- 肌肉GDF15在恢复期间下降,而血清GDF15仍然升高.
- 在两组中,运动增加了UPR/ISR标志物 (ATF4,CHOP,EIF2K3,PPP1R15A,XBP1-U,XBP1-S,EDEM1),康复后CHOP仍然升高.
- 在对照组中,eIF2α-Ser51的酸化在运动后增加,但在两组的恢复期间下降.
结论:
- 在T2D患者中,运动诱导的GDF15调节不会受到影响.
- 在T2D患者中,UPR/ISR的关键标志物也没有受到影响,与急性炼和恢复后的对照组相比.
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