希斯脱乙酶OsHDA716通过脱乙OsbZIP46来抑制大米冷却耐受性,以减少其交换活化功能和蛋白质稳定性
Ying Sun1, Zizhao Xie1, Liang Jin1
1Key Laboratory of Biorheological Science and Technology of Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, China.
The Plant cell
|January 19, 2024
概括
米植物的寒冷耐受性受到素脱乙酶OsHDA716的抑制,该酶向转录因子OsbZIP46. 这种表观遗传机制微调了植物的微调.
科学领域:
- 植物生物学 植物生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 压力反应应激反应
背景情况:
- 在全球范围内,低温显著限制了植物生长和作物产量.
- 表观遗传调节对于植物适应环境压力因素至关重要,但其在大米寒冷信号传递中的作用尚不清楚.
研究的目的:
- 阐明米 (Oryza sativa) 感冒信号和耐受性背后的表观遗传机制.
- 为了确定参与大米对低温反应的关键调节者.
主要方法:
- 调查了组织基因脱乙酶 (HDAC) OsHDA716和转录因子 OsbZIP46在大米寒冷耐受性中的作用.
- 利用了OSHDA716.16的功能丧失突变和过度表达线.
- 通过OsHDA716.6分析了OsbZIP46的相互作用和脱乙烯化.
- 评估了对下游基因 (OsDREB1A,COLD1) 和信号传导的影响.
主要成果:
- OsHDA716的功能丧失突变体表现出增强的冷却耐受性,而过度表达系则过敏.
- OsbZIP46通过激活OsDREB1A和COLD1来促进冷却耐受性,调节冷引起的流入.
- 通过OsHDA716介导的OsbZIP46脱乙烯化减少了其DNA结合能力,转录活性和蛋白质稳定性,从而降低了米饭冷却耐受性.
结论:
- 组织素脱乙酶OsHDA716通过与转录因子OsbZIP46.6相互作用和脱乙抑制了大米的寒冷耐受性.
- 染色体调节剂和转录因子之间的这种相互作用对于微调植物寒冷反应至关重要.
- 发现了一种新的机制,即HDACs通过非歇斯顿蛋白的脱乙烯化来抑制基因转录,从而影响其功能.
更多相关视频
相关概念视频
Histone Modification
13.3K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.3K
Spreading of Chromatin Modifications
8.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
8.3K
Inheritance of Chromatin Structures
6.3K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.3K
Chromatin Modification in iPS Cells
1.7K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.7K
Co-activators and Co-repressors
7.4K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.4K
Chromatin Structure Regulates pre-mRNA Processing
7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
7.0K


