由结构性调查揭示的来自甲原体古生物的谷氨胺合成酶调节机制的差异
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Marie-Caroline Müller1, Olivier N Lemaire1, Julia M Kurth2,3
1Microbial Metabolism Research Group, Max Planck Institute for Marine Microbiology, Celsiusstraße 1, 28359, Bremen, Germany.
Communications biology
|January 19, 2024
概括
古代生物具有多样化的谷氨酸合成酶 (GS) 调节. 一种类型使用2-oxoglutarate进行激活,而另一种类型使用谷氨的反抑制,揭示了各种同化策略.
科学领域:
- 生物化学 生化学
- 结构生物学 结构生物学
- 微生物生理学 微生物生理学
背景情况:
- 谷氨胺合成酶 (GS) 对于所有生命形式的氨同化至关重要.
- 与细菌和真核生物相比,了解古老的GS调节是有限的.
研究的目的:
- 阐明两种不同的古老谷氨酸合成酶的调节机制.
- 将这些机制与已知的细菌和真核生物系统进行比较和对比.
主要方法:
- 进行X射线晶体学以确定两个古老GS酶的结构.
- 在古老的GS同类物种中对残留物保存的生物信息分析.
主要成果:
- 甲热球菌 (Methanothermococcus thermolithotrophicus GS) 是通过将2-氧格酸盐结合到一个全位点来激活的,从而诱导构造变化.
- 梅瑟米可克斯 (Methermicoccus shengliensis GS) 缺乏2氧格酸盐基因,并通过通过催化循环直接抑制谷氨酸反来调节.
- 这两种调节机制,虽然在效应器和结合位点上有所不同,但在保留的活性位点决定因素上趋同.
结论:
- 考古GS表现出多种不同的调节策略,包括2-oxoglutarate激活和氨酸反抑制.
- 这些机制广泛存在,并且可能在古物种中共存.
- 尽管效应因子有差异,但GS调节的核心分子决定因素仍然存在.
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