相关实验视频

Updated: Jul 5, 2025

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

Published on: September 12, 2019

白素受体激活剂有效抑制肝细胞癌的生长

Mai Ly Thi Nguyen^1,2, Chi Pham³, Van Tran Pham^1,2

¹Vietnam Military Medical University, Hanoi, Vietnam.

Cell biochemistry and biophysics

|January 19, 2024

概括

阿迪波龙 (AdipoRon) 是一种阿迪波涅克丁受体激动剂,在体外对肝细胞癌 (HCC) 显示出显著的抗癌作用. 它的有效性与阿迪波涅克丁受体水平有关,并影响HCC细胞的攻击性.

科学领域:

肝细胞癌研究肝细胞癌研究
癌症药理学癌症药理学
分子瘤学分子瘤学

背景情况:

肝细胞癌 (HCC) 是一个重大的全球健康挑战,生存率很低.
亚迪波内克在癌症和肝脏疾病中起着保护作用;低水平 (低亚迪波内克血症) 与癌症发生率的增加有关.
阿迪波龙是一种新型阿迪波涅克丁受体激动剂,已显示出抗癌潜力,但其对HCC的影响仍未得到研究.

研究的目的:

为了研究AdipoRon在肝细胞癌 (HCC) 细胞上的体外抗癌作用.
探索AdipoRon对HCC细胞活力,迁移,入侵,殖民地形成和亡的影响.
分析潜在的信号变化,并比较不同HCC细胞系之间的AdipoRon的疗效.

主要方法:

在体外测试包括细胞活力,迁移,入侵,殖民地形成和亡.
基因和蛋白质表达分析使用RT-qPCR和西方布洛特.
对AdipoRon对Huh7和Hep3BHCC细胞系的影响进行比较分析.

主要成果:

阿迪波龙在AMPK和剂量依赖的方式在体外对HCC细胞表现出显著的抗癌活性.
与Hep3B细胞相比,Huh7细胞表现出较低的氨酸受体 (AdipoR1/2) 表达,更高的增殖和更大的攻击性.
Huh7细胞对AdipoRon治疗更敏感,显示较低的IC50和减少的生长,迁移,入侵和殖民地形成.

结论:

在体外,AdipoRon有效地抑制HCC细胞生长和侵入性.
阿迪波尼克丁受体的表达水平显著影响阿迪波龙的疗效和HCC细胞的攻击性.
这些发现突出了AdipoRon作为HCC的潜在治疗剂,受体表达是关键因素.

关键词:

这就是AMPKK.在 AdipoRon 中使用.亚迪波尼克丁氨酸是什么这是一种抗癌药物.在HCC中,HCC是HCC.这就是SEMA7A的意义.

更多相关视频

Y-90 Radioembolization and PD-1 Inhibitor as Neoadjuvant Treatment in Hepatocellular Carcinoma

Y-90 Radioembolization and PD-1 Inhibitor as Neoadjuvant Treatment in Hepatocellular Carcinoma

Published on: May 24, 2024

Non-Invasive PET/MR Imaging in an Orthotopic Mouse Model of Hepatocellular Carcinoma

Non-Invasive PET/MR Imaging in an Orthotopic Mouse Model of Hepatocellular Carcinoma

Published on: August 31, 2022

相关概念视频

GPCRs Regulate Adenylyl Cylase Activity

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Target Cell Response to Hormones

Target Cell Response to Hormones

Hormones intricately bind to receptors on the surface or within target cells, initiating a cascade of cellular responses.
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...