相关实验视频

Updated: Jul 5, 2025

Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans

Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans

Published on: February 27, 2011

在受控的短期能量赤字期间尿液稳定同位素比率的变化:一项原则证明分析

Paulina Wasserfurth¹, Frank Huelsemann², Karsten Koehler³

¹Department of Health and Sport Sciences, TUM School of Medicine and Health, Technical University of Munich, Munich, Germany.

European journal of nutrition

|January 19, 2024

概括

尿尿中的 (δ15N) 的稳定同位素比在能量不足时增加,这表明了代谢. 这项研究评估了在受控的能量赤字和运动条件下 δ15N-尿素和 Δ15N 的变化.

科学领域:

人类生理学人类生理学
营养科学营养科学
同位素地质化学同位素地质化学

背景情况:

(δ15N) 的稳定同位素比在代过程中的头发增长中.
尿液尿素同位素比例 (δ15Nurea) 可能反映了代谢变化.

研究的目的:

为了评估在短期控制的能源赤字期间δ15Nurea和Δ15N的变化.
为了研究运动与能量赤字相结合对这些标记物的影响.

主要方法:

六名从事娱乐活动的男性接受了与能量赤字 (ED) 和控制 (CON) 条件的交叉研究.
条件包括运动和没有运动的ED (ED-EX,ED+EX) 和运动和没有运动的CON (CON-EX,CON+EX).
尿样被分析为δ15Nurea; Δ15N是用食中的δ15N计算的.

主要成果:

在ED-EX条件下,与CON-EX和CON+EX相比,δ15Nurea显著增加.
在ED-EX条件下 Δ15N 呈阳性,在ED+EX,CON-EX 和 CON+EX条件下呈阴性.
在ED-EX和对照条件之间观察到Δ15N的显著差异.

结论:

δ15Nurea 和 Δ15N 是对短期能源赤字的反应.
在能量赤字期间增加的氨基酸氧化可能推动了这些变化.
偏好的14N消除可能有助于观察到的同位素转移.

关键词:

生物标志物生物标志物低能量的可用性低能量的可用性减肥的方法减肥的方法减肥的方法

更多相关视频

Using Deuterium Oxide as a Non-Invasive, Non-Lethal Tool for Assessing Body Composition and Water Consumption in Mammals

Using Deuterium Oxide as a Non-Invasive, Non-Lethal Tool for Assessing Body Composition and Water Consumption in Mammals

Published on: February 20, 2020

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx

Published on: December 20, 2016

相关概念视频

Comparative Excretory Systems

Comparative Excretory Systems

Animals have evolved different strategies for excretion, the removal of waste from the body. Most waste must be dissolved in water to be excreted, so an animal’s excretory strategy directly affects its water balance.

Overview of Nitrogen Metabolism

Overview of Nitrogen Metabolism

Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of ammonia, ammonium ions, nitrate, nitrite, or nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...

Physiology of Urine Formation

Physiology of Urine Formation

Urine formation is an essential function of the human body. It plays a critical role in maintaining homeostasis by regulating the volume and composition of body fluids. The kidneys, the primary organs involved in this process, filter blood to remove waste products and excess substances, ultimately producing urine.
Glomerular Filtration
The first stage in urine formation is glomerular filtration. Each kidney contains approximately 1 million nephrons, the functional units of filtration, with a...

Metabolic States of the Body: Fasting and Starvation

Metabolic States of the Body: Fasting and Starvation

During the initial hours of fasting, the body uses up its glycogen stores as an energy source. Once these glycogen reserves are depleted, the body begins breaking down stored triglycerides and structural proteins. During this stage, glycerol becomes a key substrate for gluconeogenesis, while free fatty acids undergo beta-oxidation to provide energy for tissues, such as skeletal muscle. In the fasting state, the body spares protein breakdown as much as possible to conserve muscle and structural...