瓦尔萨马利效应器Vm_04797与适应蛋白MdAP-2β相互作用,以操纵宿主自细胞
Yubo Sun1, Danyan Luo1, Yuerong Liu1
1State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China.
Plant physiology
|January 20, 2024
概括
瓦尔萨马利真菌使用效应剂Vm_04797通过降解MdAP-2β蛋白质来抑制果植物免疫力,抑制自并引起癌症. 这一发现揭示了真菌病原发生的一个关键机制.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 菌类遗传学 菌类遗传学
背景情况:
- 由*Valsa mali*引起的果瓦尔萨瘤对果产量产生了重大影响.
- 通过V. mali*效应器操纵宿主免疫力的分子机制尚未完全理解.
研究的目的:
- 为了识别和表征新型病毒效应因子的Valsa mali*.
- 阐明效应体Vm_04797在V. mali病原和宿主免疫抑制中的作用.
主要方法:
- *V. mali*效应器Vm_04797.7.0的识别和表征
- 在 *Nicotiana benthamiana* 中进行细胞死亡抑制的测试和在果中进行毒性测试.
- 调查Vm_04797与宿主蛋白MdAP-2β的相互作用及其在无化和自中的作用.
主要成果:
- Vm_04797,一种分泌的效应剂,抑制INF1诱导的细胞死亡,并表现出毒性功能.
- 在果植物中,Vm_04797的删除突变显示出病毒性降低.
- Vm_04797通过ubiquitination向并降解适应蛋白MdAP-2β,抑制自和宿主防御.
结论:
- Vm_04797作为一种E3泛素连酶,降解MdAP-2β以破坏自介导的植物免疫.
- 这项研究揭示了V. mali*用来克服宿主防御的新策略,为真菌病原体提供了洞察力.
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