热友生物催化剂用于将类废物单步转化为乳酸
Martina Aulitto1,2,3, Alberto Alfano4, Emanuela Maresca1
1Department of Biology, University of Naples "Federico II,", Naples, Italy.
Applied microbiology and biotechnology
|January 20, 2024
概括
这项研究表明,使用维兹曼尼亚凝结物MA-13从类废物中生产L-乳酸的可持续,单步生产. 这种微生物发酵过程有效地将废物转化为有价值的乳酸,展示了一个有前途的价值化战略.
科学领域:
- 生物技术和工业微生物学
- 可持续化学 可持续化学
- 废弃物价值化 废弃物价值化 废弃物价值化
背景情况:
- 农业食品残留物是微生物L-乳酸生产的可行的原料.
- 维兹曼尼亚凝血菌 (以前的Bacillus coagulans) 具有益生菌性质和乳酸产生能力.
- 类废物是一种丰富的农业残留物,特别是在地中海地区.
研究的目的:
- 评估使用未经处理的类废物用于L-乳酸生产的可行性.
- 开发一种可持续的,使用W.coagulans MA-13的一步发酵过程.
- 为了优化生物质降解和发酵效率.
主要方法:
- 使用未经处理的类废物作为原料.
- 采用热友酵素尾酒 (Cellic CTec2) 与W.凝血素MA-13的水解酶相结合.
- 进行批量和料批量发酵实验.
- 量化葡萄糖发酵到L-乳酸.
主要成果:
- 通过酶和微生物协同作用,增强了多达62%的生物质降解.
- 达到葡萄糖完全发酵到L-乳酸.
- 达到了 44.8 g/L 的最大 L-乳酸度.
- 从类废物中成功地证明了L-乳酸的一步生产.
结论:
- W.coagulans MA-13 作为一种有效的微生物细胞工厂,用于单阶段的L-乳酸生产.
- 具有成本效益的糖化和MA-13发酵的综合方法为农业食品废物提供了可持续的价值化途径.
- 酵素预处理为生物质加工的化学方法提供了一个可行的替代方案.
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