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在Aedes aegypti蚊子中细胞融合剂病毒DNA形式的体外表征

Mohammad Mosleh Uddin¹, Yasutsugu Suzuki², Jerica Isabel L Reyes³

¹Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 3, Matsuyama, 790-8577, Japan; Graduate School of Science and Engineering, Ehime University, Bunkyo-cho 3, Matsuyama, Ehime, Japan; Department of Biochemistry and Molecular Biology (BMB), Faculty of Life Science, Mawlana Bhashani Science and Technology University (MBSTU), Santosh, Tangail 1902, Bangladesh.

|January 20, 2024

概括

这项研究调查了非逆转录病毒内源性病毒元素 (EVE) 如何形成,发现细胞培养基对病毒DNA (vDNA) 生产产生影响. 发现揭示了多个vDNA启动部位和细胞质局部化,对于理解EVE建立至关重要.

科学领域:

病毒学病毒学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

建立非逆转录病毒内源性病毒元素 (EVE) 仍然是进化生物学中的一个重要问题.
病毒DNA (vDNA) 形式的RNA病毒被假定是EVE的前体.
细胞融合原体病毒 (CFAV),一种昆虫特异性病毒 (ISV) 在埃及甲虫,是一个罕见的非逆转录病毒RNA病毒发现为EVE.

研究的目的:

在细胞系内描述CFAV衍生的vDNA.
阐明目前病毒罕见内源化背后的机制.
了解与EVE形成相关的ISV衍生vDNA的细胞内特征.

主要方法:

在Aedes aegypti细胞系中CFAV衍生的vDNA的表征.
在不同细胞培养基条件下对vDNA生成的分析,独立于病毒复制.
在病毒基因组中检测和量化vDNA,包括单链DNA (ssDNA) 形式.

主要成果:

细胞培养基显著影响了vDNA的产生,不管CFAV的复制水平如何.
在整个病毒基因组中检测到vDNA表明了vDNA形成的多个启动点.
检测到的vDNA的很大一部分是单链 (ssDNA),在细胞质中发现的度比核更高.

结论:

细胞培养条件在产生ISV衍生的vDNA中起着至关重要的作用.
多个vDNA启动部位和ssDNA形式的存在为非逆转录病毒EVE形成提供了新的见解.
细胞质中vDNA的局部化是一个关键的细胞内特征,影响内基化潜力.

关键词:

艾得斯埃吉普提病毒 (Aedes aegypti) 是一个细胞融合代理病毒病毒.内源性病毒元素是内源性病毒元素.特定于昆虫的病毒.在RNA病毒的RNA病毒中.病毒的DNA是病毒的DNA.

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