线粒体蛋白CHCHD10抑制了NDV复制,并减少了病理变化
Xibing Yu1, Hexiang Jiang2, Jindou Li1
1State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun 130062, China.
Veterinary microbiology
|January 20, 2024
概括
纽卡斯尔病病毒 (NDV) 感染显著降低了包含10 (CHCHD10) 蛋白质水平的卷曲-卷曲-螺旋-卷曲-卷曲-螺旋域. 在小鼠中较低的CHCHD10表达与较高的病毒载量和严重疾病相关.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 纽卡斯尔病 (ND) 对全球家禽产业构成重大威胁.
- 毒性纽卡斯尔病病毒 (NDV) 的致病机制尚未完全理解.
- 对NDV感染细胞的蛋白质组分析对于理解宿主-病原体相互作用至关重要.
研究的目的:
- 调查在NDV感染期间含有10 (CHCHD10) 蛋白质的卷曲-卷曲-螺旋-卷曲-卷曲-螺旋域的作用.
- 为了确定CHCHD10在响应NDV时的表达模式.
- 评估CHCHD10表达水平对病毒载量和疾病严重性的影响.
主要方法:
- 用NDV感染的细胞进行蛋白质组分析.
- 在体内和体外检测NVD感染后的CHCHD10表达.
- 在C57BL/6小鼠中评估病毒载量和病理损伤,这些小鼠具有改变CHCHD10表达.
主要成果:
- 在NDV感染的晚期,CHCHD10蛋白水平显著下降.
- 在NDV感染后,CHCHD10表达在体内和体外显著降低.
- 在小鼠中抑制CHCHD10导致病毒载量增加和病理损伤恶化,而过度表达则产生相反的效果.
结论:
- CHCHD10对新castle病病毒的感染起着保护作用.
- 减少CHCHD10表达与增加的NDV病原性有关.
- CHCHD10可能是控制纽卡斯尔病的潜在治疗点.
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