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暴露于prothioconazole会通过诱导线粒体功能障碍和老鼠的亡来破坏卵细胞的成熟和受精

Mianqun Zhang¹, Wei Wang¹, Dandan Zhang²

¹College of Animal Science and Technology, Anhui Agricultural University, Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, Hefei, 230036, China.

Free radical biology & medicine

|January 20, 2024

概括

通过损害卵细胞质量,暴露于前列康纳 (PTC) 杀菌剂会损害女性的生育能力. 这种杀菌剂破坏了卵细胞的成熟和受精,导致生殖健康问题.

科学领域:

生殖毒理学生殖毒理学
环境科学环境科学
分子生物学分子生物学

背景情况:

蛋白甲醇 (PTC) 是一种广泛使用的三醇杀菌剂,已知对非标生物有毒作用.
关于PTC对女性生殖健康的影响,特别是卵细胞质量,成熟和受精的数据有限.

研究的目的:

调查PTC暴露对雌性小鼠卵细胞发育能力和受精能力的影响.
阐明PTC诱导的女性不孕症背后的分子机制.

主要方法:

雌性小鼠暴露于Prothioconazole (PTC) 的情况.
评估卵细胞形态,螺旋组织,染色体对齐,以及kinetochore-microtubule附件.
评估精子-卵细胞结合和关键受精相关蛋白质的动态 (Juno,皮质颗粒,Ovastacin).
单细胞转录组分析以确定受PTC影响的分子通路.

主要成果:

通过破坏形形态和染色体对齐,PTC暴露显著损害了卵细胞发育能力.
通过减少精子结合和影响与受精相关的蛋白质动态,PTC损害了卵细胞受精能力.
单细胞转录组分析揭示了PTC诱导的线粒体功能障碍,氧化应激,以及卵细胞中的亡.
暴露于PTC会导致卵细胞质量差,女性生育能力受损.

结论:

暴露于Prothioconazole (PTC) 对女性生育能力产生有毒影响.
通过分子和细胞干扰,PTC会对卵细胞质量,成熟和受精产生负面影响.
这些发现突显了与暴露于Prothioconazole (PTC) 相关的潜在生殖风险.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.在受精过程中,受精线粒体中的线粒体.卵细胞成熟的过程氧化应激是一种氧化应激.这种药物是Prothioconazole.

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