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关联CC-化学因子连接剂-2基因多态与麻风病反应

Sanjay Kumar Biswas¹, Keshar Kunja Mohanty¹, Vandana Singh¹

¹Immunology Division, ICMR-National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Dr. M. Miyazaki Marg, Agra, 282001, India.

Microbes and infection

|January 20, 2024

概括

单核酸多态性 (SNP) 在C-C基因化学因子连接物2 (CCL2) 基因中与麻风有关,特别是ENL反应. 在CCL2-2518位点的G等位基因和GG基因型增加了麻风患者红斑结节麻风 (ENL) 的风险.

科学领域:

遗传学遗传学是一个
免疫学免疫学免疫学
传染性疾病传染性疾病

背景情况:

C-C 动机化学因子连接体2 (CCL2) 是一种关键的炎症蛋白.
CCL2基因中的单核酸多态 (SNP) 可能会影响免疫反应和疾病易感性.
CCL2的变异可能会影响对Mycobacterium leprae的免疫反应.

研究的目的:

为了研究CCL2基因单核酸多态 (SNPs) 和麻风之间的关联.
确定特定的CCL2SNP是否与麻风易感或临床表现相关.

主要方法:

在975名麻风患者和357名健康对照中,使用PCR-RFLP和实时PCR与FRET进行CCL2SNP (-2518 A>G, -362 G>C, -2134 T>G, -1549 A>T) 的基因定型.
血清中CCL2,IL-12p70,IFN-γ,TNF-α和TGF-β水平的量化. 这是一个很好的方法.
细胞因子水平和CCL2基因型与麻风病状况和反应类型的相关性.

主要成果:

在麻风患者中,GCT单元型 (-2518 A>G, -362 G>C, -2134 T>G) 更常见 (P=0.04).
在 -2134 的 G 基因组在边界性麻风中占主导地位,没有反应 (P=0.03).
在CCL2 -2518的GG基因型 (P=0.008) 和G基因基因基因 (P=0.030) 与红色斑点疹 (ENL) 反应有关.
在患有-2518AA和AG基因型 (P=0.0001) 的患者中观察到血清CCL2水平升高.

结论:

在CCL2 -2518部位的G等位基因和GG基因型与麻风病中ENL反应的风险增加有关.
CCL2基因变异在麻风的免疫病原发生过程中发挥作用,特别是在ENL的发展过程中.

关键词:

这是一种CC-化学因子连接体-2的化学因子.ENL的反应 ENL的反应这是大麻风病.逆转反应是一种逆转反应.单个核酸的多态性.易感性易感性易感性

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相关概念视频

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...