跨物种的BLUF领域的多相火动态的起源
Yalin Zhou1, Siwei Tang1, Zijing Chen1
1Center for Ultrafast Science and Technology, School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.
Nature communications
|January 20, 2024
概括
蓝光中的结构变化使用黄素 (BLUF) 光受体影响光响应动态. 一个关键的WinNHin形状的百分比通过控制质子转移通路来决定弗拉文火率.
科学领域:
- 生物化学 生物化学
- 摄影生物学 摄影生物学
- 结构生物学 结构生物学
背景情况:
- 使用黄素 (BLUF) 光受体的蓝光是关键的光感应蛋白.
- 它们的光激活涉及复杂的flavin辅因子动态和质子合电子转移.
- 这些动态的特定物种变异尚未完全理解.
研究的目的:
- 研究BLUF蛋白质中不同光动态行为的结构基础.
- 为了将活性位子构造与黄素火机制相关联.
- 阐明特定形状在质子转移途径中的作用.
主要方法:
- 化托芬在特定地点内被纳入三个BLUF蛋白质 (AppA,OaPAC,SyPixD).
- 使用19F核磁共振 (NMR) 光谱对蛋白质构造的表征.
- 通过femtosecond光谱学分析flavin火动态.
主要成果:
- 使用19F NMR量化Wout,WinNHin,和WinNHout的形状.
- 确定WinNHin形状作为质子转移步骤的关键决定因素 (AppA中的一个步骤,OaPAC和SyPixD中的两个步骤).
- 黄素火率与WinNHin形状的丰度之间的相关性.
结论:
- BLUF光受体的火率是由WinNHin的百分比决定的.
- 这种形状编码了Tyr-Gln配置,阻碍了高效的质子转移.
- 在BLUF蛋白质中的结构异质性解释了物种间不同的光响应动态.
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