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评估基于酸的水泥的基因毒性,细胞毒性和生物活性
Merve Esen1, Yeliz Guven2, Mehmet Fatih Seyhan3
1Department of Pedodontics, Faculty of Dentistry, Istanbul University, Istanbul, Turkey.
BMC oral health
|January 20, 2024
概括
新的酸基水泥 (CSCs),生物MTA+和MTACem,没有表现出基因毒性或细胞毒性. 这些CSC还通过增强血管新生和骨质新生生长因子来表现出生物活性,这表明它们具有重要的脉治疗潜力.
科学领域:
- 生物材料科学 生物材料科学
- 牙科材料 牙科材料
- 恢复性牙科 恢复性牙科
背景情况:
- 基于酸的水泥 (CSCs) 越来越多地用于生命脉治疗.
- 新的CSC如Bio MTA+和MTA Cem已经出现,需要与已有的材料如Biodentine和NeoMTA一起进行评估.
研究的目的:
- 评估Bio MTA+和MTA Cem的基因毒性,细胞毒性和生物活性.
- 将这些新的CSC与Biodentine和NeoMTA进行比较.
主要方法:
- 在人体淋巴细胞中通过微核 (MN) 试验评估的基因毒性.
- 细胞毒性用人类牙纸干细胞的 WST-1 试验来评估.
- 生物活性通过ELISA测量血管性 (BMP-2,FGF-2,VEGF) 和口腔性 (ALP) 标志物来确定.
主要成果:
- 生物MTA+和MTA Cem没有显示出任何基因毒性或细胞毒性影响.
- 所有测试的CSC都增加了血管新生和骨质新生标志物水平.
- 在一些测试中,与Bio MTA+和MTA Cem相比,Biodentine和NeoMTA的BMP-2,FGF-2和VEGF水平更高.
结论:
- 生物MTA+和MTACem是安全的,没有表现出任何基因毒性或细胞毒性影响.
- 生物MTA+和MTA Cem具有生物活性潜力,促进血管新生和骨质新生因素.
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