移除转子体揭示了它们对适应性健康的贡献.
Susanne Cranz-Mileva1, Eve Reilly1, Noor Chalhoub1
1Department of Molecular Biology and Biochemistry, Rutgers, the State University of New Jersey, Piscataway, NJ, USA.
Genome biology and evolution
|January 21, 2024
概括
可转移的元素,如酵母中的Tf2逆转移子,可以使它们的宿主基因组受益. 移除Tf2损害了线粒体基因调节和宿主健康,表明适应性作用.
科学领域:
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 可转移元素 (TE) 是可在基因组内复制的移动遗传序列.
- 虽然经常被视为基因组寄生虫,但TEs也可以推动基因组进化并提供适应性益处.
- 裂变酵母 Schizosaccharomyces pombe 含有 Tf2 长端重复逆转移素家族.
研究的目的:
- 为了研究可移植元素插入在Schizosaccharomyces pombe基因组中的适应性后果.
- 确定TF2逆转移素家族在宿主健康和基因组进化中的特定作用.
- 阐明Tf2影响宿主生理学的分子机制.
主要方法:
- 从Schizosaccharomyces pombe基因组中系统地去除所有Tf2长端重复逆转移体插入物.
- 在各种生长条件下对Tf2-消去的酵母菌株进行表型分析.
- 评估基因表达,特别是线粒体基因调节,在野生类型与Tf2-ablated菌株.
主要成果:
- 废除Tf2元素对宿主Schizosaccharomyces pombe产生了显著的负面适应性影响.
- 移除Tf2导致特定线粒体基因的调节改变.
- 观察到的健身效应取决于生长条件,表明Tf2.2的特定环境贡献.
结论:
- 长端Tf2重复逆转移体对它们的Schizosaccharomyces pombe宿主体质有积极的贡献.
- Tf2元素似乎在调节宿主对代谢压力的转录反应中发挥作用,可能是通过线粒体基因调节.
- 这些发现表明Tf2逆转移子可以充当适应性元素,在响应环境线索时动态影响宿主适应性.
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