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寻找安排:细胞接触作为裂变阶段生物标志物

Chloe He¹, Neringa Karpavičiūtė², Rishabh Hariharan²

¹Wellcome/EPSRC Centre for Interventional and Surgical Sciences, University College London 43-45 Foley St, London, W1W 7TY, UK.; Department of Computer Science, University College London, 66-72 Gower St, London WC1E 6EA, UK.; AI Team, Apricity, 14 Grays Inn Rd, London WC1 X 8HN, UK..

Reproductive biomedicine online

|January 21, 2024

概括

早期人类胚胎中的三维细胞接触网络显示出改善胚胎选择的潜力. 在四细胞阶段,较高的细胞接触与更好的胚芽细胞发育相关,而在八细胞阶段,怀孕成功率更高.

科学领域:

生殖生物学生殖生物学
发育生物学是发展生物学.
生物物理学的生物物理.

背景情况:

切割阶段人类胚胎发育对于成功植入至关重要.
了解早期胚胎形态和细胞相互作用可以改善辅助生殖技术的选择.
目前胚胎评估的方法主要集中在二维形态学上.

研究的目的:

调查三维细胞接触网络与人类胚胎分裂阶段的发育潜力之间的关系.
确定来自细胞接触网络的空间生物标志物是否可以预测胚胎质量和临床结果.
探索细胞排列在四细胞阶段之外的临床相关性.

主要方法:

对胚胎镜成像数据集的回顾性分析.
从11个平面的焦点堆中,人工智能驱动的三维胚胎模型的重建.
提取和分析细胞接触网络,计算每个细胞的平均接触数.
使用未配对的t测试和接收器运行特征曲线分析的统计分析.

主要成果:

在t4时,更高的平均细胞接触与更高的爆发率和胚胎质量相关.
在t8时,更高的平均细胞接触与改善的胚胎细胞质量,生化妊娠和活产率有关.
观察到细胞安排的显著变化,八细胞胚胎显示出比四细胞胚胎更大的多样性.

结论:

三维细胞接触网络为评估超越四细胞阶段的人类植入前胚胎提供临床相关性.
这些发现表明,在第3天转移过程中,有可能改进胚胎选择技术.
需要对空间生物标志物和三维形态动力学进行进一步的研究.

关键词:

3D重建的重建是3D重建.细胞的安排是细胞的安排.细胞接触细胞接触裂纹阶段裂纹阶段胚胎学胚胎学胚胎学网络分析网络分析

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