绵羊的内分泌胰腺抵抗素:与不同饮食有关的存在和表达
Margherita Maranesi1, Elisa Palmioli2, Cecilia Dall'Aglio1
1Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, IT, Italy.
抵抗素 (RETN) 存在于绵羊胰腺的α和β细胞中,这表明它在葡萄糖调节中的作用. 羊的饮食没有影响RETN表达,但胰岛素水平因牧场质量而异.
科学领域:
- 内分泌学 在内分泌学.
- 代谢研究研究 代谢研究
- 动物科学动物科学
背景情况:
- 抵抗蛋白 (RETN) 是一种脂蛋白,与葡萄糖不耐受和胰岛素抵抗有关.
- 在各种物种的胰腺中检测到RETN,可能会影响葡萄糖平衡.
- 在阿宁牧场上养的绵羊因干旱引起的干旱而面临营养挑战.
研究的目的:
- 研究在不同饮食条件下的绵羊胰腺中抵抗素 (RETN) 的存在和表达.
- 为了确定RETN与羊胰腺的葡萄糖 (GCG) 和胰岛素 (INS) 生产细胞的同位点.
- 评估饮食质量和补充剂对羊的RETN,GCG和INS表达的影响.
主要方法:
- 免疫组织化学和免疫光学被用来检测RETN及其与GCG和INS在绵羊胰腺组织中的同定位.
- 使用定量PCR来评估RETN,GCG和INS的基因表达水平.
- 绵羊被分为三个饮食组:最大牧场开花 (MxF),最大牧场干燥 (MxD) 和实验 (Exp) 补充.
主要成果:
- 抵抗素 (RETN) 在内分泌胰腺局部化,与α (GCG) 和β (INS) 细胞共局部化.
- 在饮食组之间没有观察到RETN,GCG或INS分布和免疫染强度的显著差异.
- 虽然RETN和GCG表达保持一致,但MxF组的胰岛素 (INS) 表达明显高于Exp和MxD组.
结论:
- 抵抗素 (RETN) 存在胰腺α和β细胞中,这表明它可能在葡萄糖平衡中起调节作用.
- 羊的饮食质量和补充剂没有影响RETN或GCG表达.
- 草地营养状况显著影响胰岛素表达,突出显示饮食对胰腺功能的影响.
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