高含量增强了阳离子多糖的冷保护性能
Bruno M Guerreiro1, Patrícia Concórdio-Reis1, Helena Pericão2
1UCIBIO - Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, Caparica, Portugal; Associate Laboratory i4HB - Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, Caparica, Portugal.
International journal of biological macromolecules
|January 21, 2024
概括
富含富可的天然多糖在冷保存后显著改善细胞存活率. 这种富含糖的成分通过稳定细胞膜和破坏冰形成来提高解后活力 (PTV),优于商业冷保护剂.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 材料科学 材料科学 材料科学
背景情况:
- 低温保存对于生物样本的保存至关重要.
- 传统的冷保护剂 (CPA) 是有效的,但细胞毒性.
- 自然的多糖为冷保护提供生物相容的替代品.
研究的目的:
- 为了评估26种天然多糖的冷保护潜力.
- 确定关键的化学特征与增强的解后生存能力 (PTV) 相关联.
- 阐明富含富可糖多糖的冷保护机制.
主要方法:
- 对26种天然多糖的选,以检测它们对Vero细胞的冷保护作用.
- 分析多糖化合物的组成 (糖,尿酸,分子量).
- 主要组件分析 (PCA) 用于确定结构功能关系.
主要成果:
- 富含糖的多糖化合物显著增加了PTV (平均2.8倍,高达3.1倍).
- 最佳的冷保护需要富可 (18-35.7mol%),尿酸 (13.5-26mol%) 和高分子量 (>1MDa) 的平衡.
- 糖通过电荷独立的MW缩放机制提高了生存率,与尿酸的电荷依赖的冰破坏不同.
结论:
- 中性糖在增强细胞冷保存结果方面发挥着至关重要的作用.
- 糖可以通过独特的相互作用稳定细胞膜或调节冰的生长.
- 聚离子性不是冷保护的唯一预测因素;富含糖的多糖类提供了卓越的细胞活力.
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