来自Paenibacillus wynnii的新β-galactosidase具有工业应用的潜力
Sabine Lutz-Wahl1, Hanna Mozer1, Alena Kussler1
1Department of Biotechnology and Enzyme Science, Institute for Food Science and Biotechnology, University of Hohenheim, 70599 Stuttgart, Germany.
Journal of dairy science
|January 21, 2024
概括
来自Paenibacillus wynnii的一种新型β-galactosidase (BgaPw) 在乳糖水解和银寡糖 (GOS) 生产方面表现出卓越的性能. 这种酶提供了更好的动力学和稳定性,使其非常适合工业应用.
科学领域:
- 酶学 是一种酶学.
- 生物技术是生物技术.
- 微生物生物化学 微生物生物化学
背景情况:
- 商业β-galactosidases在基质亲和力和产品抑制方面存在局限性.
- 对于工业应用来说,识别具有更好的运动性质的酶至关重要.
研究的目的:
- 为了识别和表征一种来自Paenibacillus wynnii.的新型β-galactosidase (BgaPw).
- 评估BgaPw在乳糖水解和银寡糖 (GOS) 生产方面的潜力.
主要方法:
- 在基因序列的选中用于识别BgaPw.
- 在大肠杆菌中BgaPw的重组生成.
- 酶表征包括pH最佳值,温度稳定性和动力学研究.
- 在牛奶和合成缓冲剂中进行乳糖水解和GOS生产测定.
主要成果:
- 再组合BgaPw与野生类型相比显示出明显更高的活性.
- BgaPw在8°C和乳糖低KM时表现出极好的稳定性.
- Galactose 和葡萄糖激活了 BgaPw 活性,不同于常见的产品抑制.
- 在8°C的温度下,在72小时内实现了完整的乳糖水解,其性能优于其他β-galactosidases.
- 从药用乳糖中获得高产量的GOS (144g/L).
结论:
- Paenibacillus wynniiβ-galactosidase (BgaPw) 是一个有前途的酶,具有卓越的动力特性和稳定性.
- 在工业环境中,BgaPw为高效的乳糖水解和GOS合成提供了显著的优势.
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