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表面修饰影响人类牙上皮细胞在聚乙乙基表面的行为.

Keiju Omatsu1, Isao Yamawaki1, Yoichiro Taguchi1

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概括

对聚乙基 (PEEK) 的表面修改改善了牙上皮细胞的附着,这对于预防围植入炎至关重要. 治疗增强了PEEK上的细胞活动,PEEK是植入物支柱的替代品.

关键词:
皮质细胞是表皮细胞.周植入体炎 (peri-implantitis) 是一种发生在周植入体内的疾病.聚乙基 (PEEK) 是一种聚乙基.

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科学领域:

  • 生物材料科学 生物材料科学
  • 牙科植入物学 牙科植入物学
  • 组织工程是组织工程.

背景情况:

  • 牙上皮质附着在植入物支柱上对于预防周植入炎至关重要.
  • 聚乙基 (PEEK) 是对植入物支柱的有前途的替代品,但在生物学上是惰性的,阻碍软组织密封.
  • 为了提高PEEK的生物相容性和促进细胞融合,需要对PEEK表面进行修改.

研究的目的:

  • 研究表面处理对聚乙基 (PEEK) 的影响,以增强牙上皮细胞的附着.
  • 评估人类牙上皮细胞对改性PEEK表面的生物反应.
  • 为了确定表面的修改是否会影响PEEK的机械性能.

主要方法:

  • 聚乙基 (PEEK) 表面使用紫外线 (UV) 辐射,等离子辐射和抛光进行了修饰.
  • 分析了经过处理的PEEK的机械强度,表面形态和元素组成.
  • 在经过修改的PEEK表面上评估了人类牙上皮质细胞的粘附,增殖和整合素β4和胺素332的表达.

主要成果:

  • 表面处理并没有损害PEEK的机械强度或显著改变PEEK的表面形态.
  • 所有的处理方法都增加了表面的氧含量和可湿性.
  • 人类牙上皮细胞粘附,增殖和关键粘附蛋白的表达 (integrin β4,laminin 332) 在经过修改的PEEK表面上显著增强.

结论:

  • 使用紫外线照射,等离子体或抛光对PEEK表面进行修改,可以增强其生物特性.
  • 这些修改促进了牙上皮细胞的活动,这表明PEEK植入物支柱周围软组织密封的潜力有所提高.
  • 修改后的PEEK显示出作为牙植入物支架的生物相容材料的潜力,有助于预防周 implantitis.