在种子培养中研究细菌种群动态,用于从合成废水中减少氨
Raghupatruni Lakshmi Manasa1, Alka Mehta2
1Department of Integrative Biology, School of Biosciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, 632014, India.
World journal of microbiology & biotechnology
|January 21, 2024
概括
这项研究丰富了无氧和半无氧细菌700天,确定了废水中总去除的关键物种. 观察到增强的细菌生长和效率,为试点规模的生物反应堆铺平了道路.
科学领域:
- 环境微生物学 环境微生物学
- 废水处理技术 废水处理技术
- 气循环生物修复 气循环生物修复
背景情况:
- 氨是水生环境中主要的有毒污染物,需要有效的废水处理.
- 使用无氧和半无氧细菌的生物处理系统对于去除至关重要.
- 识别和维持用于废水处理的细菌群落,特别是不可培养的化学基质营养菌,仍然是一个挑战.
研究的目的:
- 为了丰富和识别参与废水中总去除的细菌群体.
- 为了研究这些细菌在长时间内的可持续性和丰富性.
- 评估开发高效生物处理系统的潜力.
主要方法:
- 使用合成废水700天丰富无毒和半无毒细菌.
- 通过16S rRNA基因聚合酶链反应 (PCR) 和DNA测序进行分子鉴定.
- 使用定量PCR (qPCR) 量化化莫利托丰度的量化.
主要成果:
- 确定了Nitrosomonas europaea作为一种完整的氨氧化剂,与未培养的Nitrospira和anammox细菌一起.
- 观察到anammox和comammox细菌的共存,促进更长的细菌生长时间.
- 检测到像Pseudomonas denitrificans和Aminobacter aminovorans这样的脱剂,表明部分脱.
结论:
- 半无毒和无毒细菌的丰富与化,comammox,anammox和脱细菌的增长加快.
- 增强的细菌生长和效率表明,小型试点级生物反应器的潜力是为了完全去除.
- 该研究强调了成功的长期丰富和识别多样化的细菌群落,以有效处理废水.
相关概念视频
Bacterial Growth Curve
The bacterial growth curve is a fundamental concept in microbiology that describes the dynamics of bacterial population growth in a closed system with controlled environmental conditions, such as temperature and nutrient availability. This curve is divided into four distinct phases: lag, log (exponential), stationary, and death phases, each reflecting a unique stage of bacterial adaptation and growth. During the lag phase, bacteria acclimate to their surroundings by synthesizing essential...
Microbial Growth Measurement: Indirect Methods
Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
Microbial Wastewater Treatment
Microbial communities in aquatic ecosystems play a key role in the natural breakdown of contaminants introduced through domestic and industrial effluents. Acting as biological catalysts, these microbes change and mineralize a wide range of organic and inorganic pollutants under different redox conditions.In oxygen-rich surface waters, aerobic heterotrophs lead organic matter breakdown, using oxygen as the terminal electron acceptor to efficiently oxidize substrates to carbon dioxide and water.
Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...


