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适应性实验室益生菌向氧化压力的进化使用基于微流体的平台

Ann V Nguyen¹, Mohammad Yaghoobi¹, Shiying Zhang²

¹Department of Food Science, College of Agricultural and Life Sciences, Cornell University, Stocking Hall, Ithaca, NY, 14853, USA.

Small (Weinheim an der Bergstrasse, Germany)

|January 22, 2024

概括

使用一种新的微流体芯片增强的适应性实验室进化 Lacticaseibacillus rhamnosus GG GG.

科学领域:

微生物学微生物学
生物技术是生物技术.
合成生物学合成生物学

背景情况:

适应性实验室进化 (ALE) 增强了细菌对氧化应激的抵抗力.
微流体平台为ALE提供了传统批量培养的高效和经济的替代方案.
现有的微流体ALE系统面临着细胞通过和空间控制方面的挑战.

研究的目的:

为ALE开发和评估一种新的微流体芯片进化 (EVoc) 系统.
通过使用 EVoc 平台,提高 Lacticaseibacillus rhamnosus GG 的氧化应激耐受性.
在长时间暴露于H2O2下,研究L. rhamnosus GG的适应机制和遗传变化.

主要方法:

使用微流体EVoc设计,具有渐进的H2O2梯度.
对L. rhamnosus GG进行长期ALE (长达72小时)
分析了适应性菌株形态,基因表达,并进行了全基因组测序.

主要成果:

成功进化了L. rhamnosus GG的适应性菌株,表现出增加的H2O2耐受性.
经过ALE后,在增加H2O2度 (1-3mM) 方面表现出逐步增长的增强.
与野生类型相比,在适应菌株中观察到不同的形态和基因表达特征.
在适应菌株中确定了omega-amidase基因中的单个核酸突变.

结论:

微流体EVoc平台对ALE有效,增强了细菌的氧化应激抵抗力.
ALE显著提高了L. rhamnosus GG在过氧化的存在下耐受和生长的能力.
基因适应,包括omega-amidase的突变,是增强压力耐受性的基础.

关键词:

适应性实验室进化的演变.适应性菌株是一种适应性菌株.在芯片上的实验室氧化应激是一种氧化应激.益生菌是一种益生菌.

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