双功能组织工程复合结构用于骨再生:铜和纤维素的作用
Azam Bozorgi1,2,3, Mozafar Khazaei3, Maryam Bozorgi3
1Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
概括
铜注入的支架和纤维素封装增强骨再生,通过促进骨质生成和血管生成在介质干细胞. 这种双功能方法改善了骨缺陷的愈合 in vivo.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 双功能组织工程支架对于骨再生至关重要,需要同时促进骨质生成和血管生成.
- 嵌入金属离子的支架和纤维素封装提供对离子/生长因子释放的可调节控制,并且具有成本效益和无毒性.
研究的目的:
- 调查铜-纳米酸/奇托/凝 (Cu-nHA/Cs/Gel) 支架和纤维素封装对沃顿果介质干细胞 (WJMSCs) 的骨质和血管分化的影响.
- 评估这些结构的体外和体外骨再生能力.
主要方法:
- 在Cu-nHA/Cs/Gel支架上进行了合成和特征化.
- WJMSCs被隔离,特征,并播种在支架上,有或没有纤维素封装.
- 通过RT-PCR,西部斑点和阿利沙林红色染色来评估骨质原生和血管原生差异.
- 脚手架植入于老鼠的形缺陷中,然后进行组织学分析 (H&E,马森三色,CD31染色).
主要成果:
- Cu-nHA/Cs/Gel支架表现出适合细胞生长的物理化学和生物特性.
- 铜和纤维素都显著增加了WJMSCs中关键骨质性 (ALP,RUNX2,OCN,COLI) 和血管性 (VEGF,HIF1α) 标志物的表达.
- 在体内,支架具有生物相容性,并且与宿主组织结合得很好,显示出增强的原凝结,矿化和血管形成.
- 纤维素封装导致更高的原/细胞密度,免疫细胞透和骨形形成,CD31阳性细胞增加.
结论:
- 将铜纳入支架和将WJMSC封装在纤维素中是促进骨质生成和血管生成的有效策略.
- 这种综合方法通过引导参与骨形成和血管化的细胞信号通路,显著增强骨再生.
- 开发的双功能结构有望改善骨缺陷修复应用.
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