从黄蜂中分离高价值化合物,使用优化的顺序提取程序
Ana I Paniagua-García1, David Ruano-Rosa2, Rebeca Díez-Antolínez1
1Centre of Biofuels and Bioproducts, Agricultural Technological Institute of Castilla y León, Villarejo de Órbigo, E-24358 León, Spain.
Antioxidants (Basel, Switzerland)
|January 22, 2024
概括
这项研究优化了使用甲醇-二甲混合物的化合物提取,最大限度地恢复了α-酸,β-酸,和. 软树脂表现出最高的抗氧化活性.
科学领域:
- 农业和食品化学 农业和食品化学
- 自然产品化学 自然产品化学
- 过程化学 过程化学
背景情况:
- 蜂含有宝贵的化合物,如α-酸,β-酸,和,具有多样化的应用.
- 有效的提取和分离方法对于将这些高价值化合物从麻基质中分离出来至关重要.
- 现有的方法可能缺乏优化,以最大限度地提高不同类别化合物的回收.
研究的目的:
- 开发和优化从蜂颗粒中获得高价值化合物的连续提取和分离过程.
- 为了最大限度地提高α-酸,β-酸,和的恢复产量.
- 为了评估提取的部分的抗氧化特性.
主要方法:
- 对十种有机溶剂进行选,以确定固体-液体提取效率.
- 使用甲醇-二甲混合物优化提取变量 (溶剂组成,温度,时间).
- 分成软树脂,硬树脂和废固体.
- 在六种蜂品种中验证优化条件.
- 使用铁减少抗氧化功率 (FRAP) 和激素清除活性 (EC50) 测试抗氧化特性.
主要成果:
- 在室温下以19.7%的甲醇进行89分钟的优化提取,得到了很高的回收率:86.57%的α-酸,89.14%的β-酸 (软树脂),78.48%的xantohumol (硬树脂) 和67.10%的phenolics (消耗固体).
- 优化的流程证明了在六种不同的蜂品种中成功验证.
- 软树脂分离物表现出最高的抗氧化活性,FRAP值为3.91 ± 0.10 g AAE/100 g,EC50值为0.10 ± 0.01 mg/mL.
结论:
- 开发的顺序提取和分离过程是有效的,用于从蜂中分离有价值的化合物.
- 优化的方法提供了高产量,并证明了在各种蜂品种中广泛适用.
- 这些发现支持大规模工业生产具有显著抗氧化特性的蜂衍生化合物的潜力.
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