罗基诺林基通过其抗氧化能力在4°C储存期间改善雄羊精子质量
Zhendong Zhu1, Wenjia Li1, Qitai Yang1
1College of Animal Science and Technology, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, China.
Antioxidants (Basel, Switzerland)
|January 22, 2024
概括
罗基诺林 (PQQ) 补充剂通过减少氧化应激和维持线粒体功能来改善冷的公羊精子质量. 这在保存期间提高了精子的运动性,膜完整性和受精能力.
科学领域:
- 生殖生物学 生殖生物学
- 氧化压力研究研究 氧化压力研究
- 生物化学 生物化学
背景情况:
- 精子运动对于受精至关重要,但体外保存会产生过多的活性氧物种 (ROS) 损害精子功能.
- 在冷藏期间保持精子质量对于人工授精和生殖技术至关重要.
研究的目的:
- 为了研究罗基诺林 (PQQ) 在改善冷公羊精子质量的有效性.
- 阐明PQQ在精子保存过程中对氧化损伤的保护机制.
主要方法:
- 雄羊的精液被稀释在Tris-酸-葡萄糖 (TCG) 中介中,其PQQ度各不相同 (0-10,000 nM),并存储在4°C.
- 评估了精子运动性,血和体完整性,线粒体膜潜力,ROS,马隆迪甲 (MDA),超氧化物失调酶 (SOD) 和ATP水平.
- 评估了MT-ND1和MT-ND6的蛋白质表达,精子容量和透明区结合能力.
主要成果:
- PQQ显著改善了雄羊精子的运动性,血膜完整性和体完整性 (p < 0.05).
- PQQ治疗增加了线粒体膜潜力和ATP水平,同时降低了ROS和MDA水平.
- PQQ提升了MT-ND1和MT-ND6蛋白质的表达,减少了ROS对这些蛋白质的损伤,并增强了精子电容和 zona pellucida结合.
结论:
- 通过降低ROS水平和减轻氧化损伤,PQQ保护冷却的公羊精子质量.
- PQQ维持精子线粒体功能,并在保存期间增强受精潜力.
- 最佳的PQQ度 (100nM) 显著改善了精子的关键参数和受精能力.
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